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Showing 1-30 of 327 results for "P2308" within Papers
Palraj Kalimuthu et al.
Analytical chemistry, 92(19), 13110-13117 (2020-09-01)
Proteases are often used as biomarkers of many pathologies as well as of microbial contamination and infection. Therefore, extensive efforts are devoted to the development of protease sensors. Some applications would benefit from wireless monitoring of proteolytic activity at minimal
Diego J Páez-Moscoso et al.
Nature communications, 13(1), 1067-1067 (2022-02-27)
Telomerase reverse transcriptase (TERT) and the noncoding telomerase RNA (TR) subunit constitute the core of telomerase. Additional subunits are required for ribonucleoprotein complex assembly and in some cases remain stably associated with the active holoenzyme. Pof8, a member of the
Aida Karray et al.
Molecules (Basel, Switzerland), 25(22) (2020-11-26)
The main objective of the current study was the extraction, purification, and biochemical characterization of a protein protease inhibitor from Conyzadioscoridis. Antimicrobial potential and cytotoxic effects were also examined. The protease inhibitor was extracted in 0.1 M phosphate buffer (pH
Rikuhide Koma et al.
Physiological reports, 9(5), e14769-e14769 (2021-03-03)
Mitochondria play a principal role in metabolism, and mitochondrial respiration is an important process for producing adenosine triphosphate. Recently, we showed the possibility that the muscle-specific protein myoglobin (Mb) interacts with mitochondrial complex IV to augment the respiration capacity in
Shai Ofek et al.
BMC biology, 19(1), 84-84 (2021-04-25)
The dorsal domain of the neural tube is an excellent model to investigate the generation of complexity during embryonic development. It is a highly dynamic and multifaceted region being first transiently populated by prospective neural crest (NC) cells that sequentially
Changyi Ji et al.
Autophagy, 15(7), 1199-1213 (2019-02-13)
A major cellular catabolic pathway in neurons is macroautophagy/autophagy, through which misfolded or aggregation-prone proteins are sequestered into autophagosomes that fuse with lysosomes, and are degraded. MAPT (microtubule-associated protein tau) is one of the protein clients of autophagy. Given that
Athanasia Mizi et al.
Methods (San Diego, Calif.), 170, 33-37 (2019-07-10)
Genome organization is now understood to be tightly linked to all genomic functions. Thus, the high-resolution mapping of higher-order chromosomal structures via 3C-based approaches has become an integral tool for studying transcriptional and cell cycle regulation, signaling effects or disease
Xinping Lin et al.
Yeast (Chichester, England), 29(11), 467-474 (2012-10-16)
Red yeasts hold great promise in the production of microbial lipids and carotenoids. Genetic study of red yeasts has attracted much attention; however, rapid amplification of genes from red yeast samples remains technically challenging. Here a highly efficient method for
Jimmy Su et al.
Journal of biomedical materials research. Part A, 106(9), 2448-2462 (2018-04-18)
Hydrogels, highly-hydrated crosslinked polymer networks, closely mimic the microenvironment of native extracellular matrix (ECM) and thus present as ideal platforms for three-dimensional cell culture. Hydrogels derived from tissue- and organ-specific decellularized ECM (dECM) may retain bioactive signaling cues from the
Heejeong Kim et al.
International journal of molecular sciences, 21(17) (2020-08-29)
Selective autolysosomal degradation of damaged mitochondria, also called mitophagy, is an indispensable process for maintaining integrity and homeostasis of mitochondria. One well-established mechanism mediating selective removal of mitochondria under relatively mild mitochondria-depolarizing stress is PINK1-Parkin-mediated or ubiquitin-dependent mitophagy. However, additional
Gerhild Zauner et al.
Methods in molecular biology (Clifton, N.J.), 951, 113-127 (2013-01-09)
Liquid chromatography-tandem stage mass spectrometry of glycopeptides is a powerful tool for the site-specific glycosylation analysis of glycoproteins. Using fetuin as a model substance, we describe a protocol for glycopeptide dissection using nonspecific proteolysis by proteinase K. Proteolysis is achieved
Amol Kanampalliwar et al.
Frontiers in microbiology, 11, 1838-1838 (2020-09-29)
Vibrio cholerae is an autochthonous inhabitant of the aquatic environment. Several molecular methods have been used for typing V. cholerae strains, but there is no proper database for such scheme, including multilocus sequence typing (MLST) for V. cholerae O1 and
Marcus Nordgren et al.
Autophagy, 11(8), 1326-1340 (2015-06-19)
Peroxisomes are ubiquitous cell organelles essential for human health. To maintain a healthy cellular environment, dysfunctional and superfluous peroxisomes need to be selectively removed. Although emerging evidence suggests that peroxisomes are mainly degraded by pexophagy, little is known about the
Han Sun et al.
Bing du xue bao = Chinese journal of virology, 28(4), 409-413 (2012-09-18)
In order to explore the potential influences of the disulfide bridge on the physical and chemical properties of PrP protein, the expressed recombinant human wild-type PrP protein was purified for using in an established redox process for the reduction and
Mouse primordial germ cells: isolation and in vitro culture.
Patricia A Labosky et al.
Methods in molecular biology (Clifton, N.J.), 461, 187-199 (2008-11-26)
Laura Klasek et al.
The Plant cell, 32(12), 3884-3901 (2020-10-24)
Posttranslational protein targeting requires chaperone assistance to direct insertion-competent proteins to integration pathways. Chloroplasts integrate nearly all thylakoid transmembrane proteins posttranslationally, but mechanisms in the stroma that assist their insertion remain largely undefined. Here, we investigated how the chloroplast chaperonin
Wen Si Hu et al.
Scientific reports, 10(1), 21090-21090 (2020-12-05)
Bacteria can form biofilms, complex microbial communities protected from environmental stress, on food contact surfaces. Brassicaceae plant has been shown to contain bioactive compounds with antimicrobial activities. The objective of this study was to evaluate the synergistic effects of Brassicaceae
Todd W Osmundson et al.
Molecular ecology resources, 13(1), 66-74 (2012-11-06)
The ubiquity, high diversity and often-cryptic manifestations of fungi and oomycetes frequently necessitate molecular tools for detecting and identifying them in the environment. In applications including DNA barcoding, pathogen detection from plant samples, and genotyping for population genetics and epidemiology
Richard P Davis et al.
Nature protocols, 3(10), 1550-1558 (2008-09-20)
The first step in the generation of genetically tagged human embryonic stem cell (HESC) reporter lines is the isolation of cells that contain a stably integrated copy of the reporter vector. These cells are identified by their continued growth in
Sarah McCarrick et al.
International journal of nanomedicine, 16, 5895-5908 (2021-09-04)
Gold nanoparticles (AuNPs) have the potential to be used in various biomedical applications, partly due to the inertness and stability of gold. Upon intravenous injection, the NPs interact with the mononuclear phagocyte system, first with monocytes in the blood and
Markus Maerker et al.
Nature communications, 12(1), 5482-5482 (2021-09-18)
Rotating cilia at the vertebrate left-right organizer (LRO) generate an asymmetric leftward flow, which is sensed by cells at the left LRO margin. Ciliary activity of the calcium channel Pkd2 is crucial for flow sensing. How this flow signal is
Beda Mühleisen et al.
Archives of dermatology, 148(6), 697-703 (2012-04-18)
To evaluate chromosomal instability at 9p21-22 with p16 protein expression in organ transplant recipients (OTRs) compared with immunocompetent patients with squamous cell carcinoma (SCC). In a select population of intraepithelial and subsequent invasive SCC from the same anatomic region of
Magdaline Costa et al.
Nature protocols, 2(4), 792-796 (2007-04-21)
The ability to genetically modify human embryonic stem cells (HESCs) will be critical for their widespread use as a tool for understanding fundamental aspects of human biology and pathology and for their development as a platform for pharmaceutical discovery. Here
Anna Goralczyk et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 31(8), 3251-3266 (2017-04-19)
Transient receptor potential (TRP) channels are polymodal cell sensors responding to diverse stimuli and widely implicated in the developmental programs of numerous tissues. The evidence for an involvement of TRP family members in adipogenesis, however, is scant. We present the
Micaela Pivato et al.
PloS one, 7(12), e50027-e50027 (2012-12-29)
The aggregation of α-synuclein into amyloid fibrils constitutes a key step in the onset of Parkinson's disease. Amyloid fibrils of α-synuclein are the major component of Lewy bodies, histological hallmarks of the disease. Little is known about the mechanism of
Yakubu Princely Abudu et al.
The Journal of cell biology, 220(8) (2021-05-27)
Mitophagy is the degradation of surplus or damaged mitochondria by autophagy. In addition to programmed and stress-induced mitophagy, basal mitophagy processes exert organelle quality control. Here, we show that the sorting and assembly machinery (SAM) complex protein SAMM50 interacts directly
Johannes Hedman et al.
Forensic science international. Genetics, 53, 102491-102491 (2021-03-29)
Efficient sampling with swabs is crucial for optimal forensic DNA analysis. The DNA recovery is determined by the skill of the practitioner and the compatibility between the applied swab and the surface. Here we investigate the impact of swabbing technique
Tugba Kilic et al.
Biofouling, 33(4), 352-367 (2017-04-21)
The ability of Aeribacillus pallidus E334 to produce pellicle and form a biofilm was studied. Optimal biofilm formation occurred at 60 °C, pH 7.5 and 1.5% NaCl. Extra polymeric substances (EPS) were composed of proteins and eDNA (21.4 kb). E334 formed
A glucokinase-linked sensor in the taste system contributes to glucose appetite.
Chometton, et al.
Molecular Metabolism, 64, 101554-101554 (2022)
Sasmita Panda et al.
Frontiers in microbiology, 9, 2687-2687 (2018-11-30)
Staphylococcus haemolyticus is the second most frequently isolated CoNS from ocular infections and human blood cultures. In this study, we examined 18 ocular S. haemolyticus isolates for their capacity to form biofilm and conducted detachment assay to determine the composition
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