P3126

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Showing 1-26 of 26 results for "P3126" within Papers

Use of molecularly imprinted polymers in a biotransformation process.

L Ye et al.

Biotechnology and bioengineering, 64(6), 650-655 (1999-07-23)

Molecularly imprinted polymers are highly stable and can be sterilised, making them ideal for use in biotransformation process. In this communication, we describe a novel application of molecularly imprinted polymers in an enzymatic reaction. The enzymatic condensation of Z-L-aspartic acid

Cis/trans conformational equilibrium across the N-methylphenylalanine- N-methylphenylalanine peptide bond of arginine vasopressin analogs.

E Trzepałka et al.

The journal of peptide research : official journal of the American Peptide Society, 63(4), 333-346 (2004-04-23)

Two cyclic analogs of vasopressin, -Pro-Arg-Gly-NH(2) (1) and -Pro-Arg-Gly-NH(2) (2) were synthesized by the solid phase method. Their structure was determined in aqueous solution by two-dimensional NMR techniques and simulated annealing algorithm from an extended template in X-PLOR. The total

Improving lipase enantioselectivity in organic solvents by forming substrate salts with chiral agents.

J S Shin et al.

Biotechnology and bioengineering, 69(5), 577-583 (2000-07-18)

We recently demonstrated (J Am Chem Soc 121:3334-3340, 1999) that enzymatic enantioselectivity in organic solvents can be markedly enhanced by temporarily enlarging the substrate via salt formation. In the present study, this approach was expanded by finding that, in addition

Inhibition of programmed cell death by catalase and phenylalanine methyl ester.

G H Little et al.

Comparative biochemistry and physiology. Comparative physiology, 105(1), 79-83 (1993-05-01)

1. Programmed cell death proceeds by an unknown mechanism which results in characteristic morphological changes known as apoptosis. 2. We have proposed that, in hormone-induced apoptosis, cell death may be the result of an attack of cells destined to die

Aromatic amino acid methyl ester analogs form quinonoidal species with Dopa decarboxylase.

P S Moore et al.

FEBS letters, 412(1), 245-248 (1997-07-21)

This study reports for the first time that binding of aromatic methyl ester analogs to Dopa decarboxylase in the native and inactive nicked forms causes the appearance of a dead-end quinonoidal species absorbing at 500 nm, in addition to an

Peptide synthesis of aspartame precursor using organic-solvent-stable PST-01 protease in monophasic aqueous-organic solvent systems.

Shotaro Tsuchiyama et al.

Biotechnology progress, 23(4), 820-823 (2007-05-08)

The PST-01 protease is a metalloprotease that has zinc ion at the active center and is very stable in the presence of water-soluble organic solvents. The reaction rates and the equilibrium yields of the aspartame precursor N-carbobenzoxy-L-aspartyl-L-phenylalanine methyl ester (Cbz-Asp-Phe-OMe)

Screening, purification, and identification of the enzyme producing N-(L-alpha-L-aspartyl)-L-phenylalanine methyl ester from l-isoasparagine and L-phenylalanine methyl ester.

Ikuo Kira et al.

Journal of bioscience and bioengineering, 108(3), 190-193 (2009-08-12)

Screening was carried out for microorganisms able to produce N-(l-alpha-l-aspartyl)-l-phenylalanine methyl ester [APM] from l-isoasparagine and l-phenylalanine methyl ester hydrochloride. Of the 422 strains examined, 44 strains belonging to the family Enterobacteriaceae were found to produce APM. The enzyme catalyzing

Transport and signaling via the amino acid binding site of the yeast Gap1 amino acid transceptor.

Griet Van Zeebroeck et al.

Nature chemical biology, 5(1), 45-52 (2008-12-09)

Transporter-related nutrient sensors, called transceptors, mediate nutrient activation of signaling pathways through the plasma membrane. The mechanism of action of transporting and nontransporting transceptors is unknown. We have screened 319 amino acid analogs to identify compounds that act on Gap1

Antileukemic activity of phenylalanine methyl ester (PME): a lysosomotropic peptide methyl ester.

C S Rosenfeld

Stem cells (Dayton, Ohio), 12(2), 198-204 (1994-03-01)

The antileukemic activities of the lysosomotropic compounds, such as phenylalanine methyl ester (PME), have received little attention. In this study, a 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay was used to investigate the antileukemic activity of PME. Leukemic specimens from untreated patients that

Synergism between apple procyanidins and lysosomotropic drugs: potential in chemoprevention.

Nikolaus Seiler et al.

Anticancer research, 26(5A), 3381-3385 (2006-11-11)

Procyanidins are apple constituents with potential in colon cancer chemoprevention. Human colon cancer derived metastatic cells (SW620), growing under standardized conditions, were exposed to procyanidins and lysosomotropic compounds. Growth, apoptosis and lysosomal integrity was determined using published methods. Lysosomotropic drugs

Clinically applicable bulk isolation of blood CD34+ cells for autografting in children.

Y Kawano et al.

Bone marrow transplantation, 22(10), 1011-1017 (1998-12-16)

CD34+ cells were purified in bulk from apheresis-collected cells of children with cancer using monoclonal antibody (MoAb) and magnetic beads (Baxter ISOLEX system). To improve the purity of the final product for possibly better tumor cell purging and to make

A unique enzyme from Saccharothrix sp. catalyzing D-amino acid transfer.

Y Watanabe et al.

Biochimica et biophysica acta, 1337(1), 40-46 (1997-01-04)

A newly isolated actinomycete belonging to Saccharothrix sp. was found to produce a unique enzyme catalyzing D-amino acid transfer. The enzyme, which was tentatively named D-amino acid transferase, was purified 2600-fold to electrophoretic homogeneity and the molecular mass was 41

A new application of molecularly imprinted materials.

L Ye et al.

Journal of molecular recognition : JMR, 11(1-6), 75-78 (1999-03-17)

We have studied the possibility of shifting a thermodynamically unfavourable enzymatic equilibrium towards product formation via the addition of a highly specific adsorbent. The commercially interesting enzymatic condensation of Z-L-aspartic acid with L-phenylalanine methyl ester to the sweetener aspartame was

Purified CD34+ Lin- Thy+ stem cells do not contain clonal myeloma cells.

Y Gazitt et al.

Blood, 86(1), 381-389 (1995-07-01)

High-dose therapy with autologous marrow or peripheral blood stem cell (PBSC) rescue has been extensively applied in the treatment of multiple myeloma (MM) patients during the past 10 years resulting in improved event-free and overall survival when compared with standard

Synthesis, characterization, X-ray crystallography, and cytotoxicity of a cymantrene keto carboxylic acid for IR labelling of bioactive peptides on a solid support.

Harmel W Peindy N'Dongo et al.

Journal of inorganic biochemistry, 102(12), 2114-2119 (2008-09-18)

Cym-CO-CH2-CH2-COOH was prepared in good yield by Friedel-Crafts reaction of cymantrene (Cym, CpMn(CO)3) with succinic anhydride for the IR labelling of peptides and fully characterized, including an X-ray structure analysis (monoclinic space group P2(1)/n, a=5.727(3)A, b=19.865(9)A, c=10.518(5)A, beta=91.211(9) degrees). The

Spectrum of toxicities of amino acid methyl esters for myeloid cells is determined by distinct metabolic pathways.

D L Thiele et al.

Blood, 79(4), 964-971 (1992-02-15)

L-leucine methyl ester (Leu-OMe), Leu-Leu-OMe, Phe-OMe, and Glu-(OMe)2 are toxic to mononuclear phagocytes (M phi) and neutrophils. In the present studies, the mechanism of this toxicity was examined. A concentration of NH4Cl known to neutralize lysosomal pH and to block

Promotion of the formation of phenylalanine-thymine cross-linked products by free radicals formed during carbon tetrachloride activation with benzoyl peroxide in model systems. A mass spectrometric structural study.

G D Castro et al.

Research communications in molecular pathology and pharmacology, 98(1), 85-90 (1998-01-22)

Reaction mixtures containing phenylalanine methyl ester and thymine in pure carbon tetrachloride in the presence of benzoyl peroxide produced trichloromethyl and trichloromethylperoxyl free radicals which via hydrogen abstraction reactions sparked the formation of phenylalanine-thymine adducts, whose structures were elucidated by

Demethylation kinetics of aspartame and L-phenylalanine methyl ester in aqueous solution.

R D Skwierczynski et al.

Pharmaceutical research, 10(8), 1174-1180 (1993-08-01)

The kinetics of demethylation of aspartame and L-phenylalanine methyl ester were studied in aqueous solution at 25 degrees C over the pH range 0.27-11.5. The pseudo-first-order rate constant for aspartame was resolved into individual contributions from methyl ester hydrolysis and

Biologic effects of the adoptive transfer of cells depleted of monocytes with L-phenylalanine methyl ester.

P L Triozzi et al.

Immunopharmacology, 28(1), 39-45 (1994-07-01)

Monocytes macrophages have negative regulatory effects on many immunologic responses. Depletion of monocytes from peripheral blood using the lysosomotropic agent, L-phenylalanine methyl ester (PME), has been shown to improve lymphokine activated killer (LAK) cell expansion in vitro. A pilot study

Reaction of ADP with amino acid methyl esters mediated by trimethylsilyl chloride.

Hua Fu et al.

Chemical communications (Cambridge, England), (1)(1), 134-135 (2003-03-04)

Reaction of ADP with amino acid methyl esters mediated by trimethylsilyl chloride in pyridine produced adenosine 5'-phosphoramidates in good yields under mild conditions, it is interesting that nucleophilic attack of amino acid methyl esters only occurred on alpha-phosphorus of ADP.

Developing novel hCT derived cell-penetrating peptides with improved metabolic stability.

Robert Rennert et al.

Biochimica et biophysica acta, 1758(3), 347-354 (2005-12-29)

Many promising therapeutics are currently awaiting their clinical application. Due to their low capability of cell membrane crossing, these compounds do not reach their site of action. One way to overcome this problem might be the fusion of these agents

Potential of phenylalanine methylester as a bone marrow purging agent.

C S Rosenfeld

Blood, 80(9), 2401-2405 (1992-11-01)

Phenylalanine methylester (PME), a lysosomotropic compound can be used to deplete monocytes and myeloid cells from peripheral blood and bone marrow (BM). The potential of PME for purging leukemic cells from BM was investigated using U937 and HL-60 cell lines

N-methyl phenylalanine-rich peptides as highly versatile blood-brain barrier shuttles.

Morteza Malakoutikhah et al.

Journal of medicinal chemistry, 53(6), 2354-2363 (2010-02-23)

Here we studied the capacity of N-MePhe-(N-MePhe)(3)-CONH(2), Cha-(N-MePhe)(3)-CONH(2), and 2Nal-(N-MePhe)(3)-CONH(2) to carry various drugs (cargos) in in vitro blood-brain barrier (BBB) models in order to determine the versatility of these peptides as BBB-shuttles for drug delivery to the brain. Using

Highly selective bradykinin agonists and antagonists with replacement of proline residues by N-methyl-D- and L-phenylalanine.

S Reissmann et al.

Journal of medicinal chemistry, 39(4), 929-936 (1996-02-16)

For further studies on the structural and conformational requirements of positions 2,3, and 7 in the bradykinin sequence, we replaced the proline residues by the more hydrophobic and conformationally restricted N-methyl-L- and D-phenylalanine (NMF). The biological activities of the new

Enzyme activity evaluation of organic solvent-treated phenylalanine ammonia lyase.

Andrew J Quinn et al.

Biotechnology progress, 27(6), 1554-1560 (2012-01-12)

The direct one-step synthesis of L-phenylalanine methyl ester in an organic-aqueous biphasic system using phenylalanine ammonia lyase (E.C.4.3.1.5, PAL) containing Rhodotorula glutinis yeast whole cells was reported earlier. We report here further optimization of this biotransformation using isolated PAL, when

Asymmetric conjugate reductions with samarium diiodide: asymmetric synthesis of (2S,3R)- and (2S,3S)-[2-2H,3-2H]-leucine-(S)-phenylalanine dipeptides and (2S,3R)-[2-(2)H,3-2H]-phenylalanine methyl ester.

Stephen G Davies et al.

Organic & biomolecular chemistry, 3(8), 1435-1447 (2005-04-14)

The highly diastereoselective samarium diiodide and D(2)O-promoted conjugate reduction of homochiral (E)- and (Z)-benzylidene and isobutylidene diketopiperazines (E)-5,7 and (Z)-6,8 has been demonstrated. This methodology allows the asymmetric synthesis of methyl (2S,3R)-dideuteriophenylalanine 27 in > or = 95% de and

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