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Showing 1-30 of 144 results for "P3655" within Papers
Michael Zabolocki et al.
Nature communications, 11(1), 5550-5550 (2020-11-05)
The capabilities of imaging technologies, fluorescent sensors, and optogenetics tools for cell biology are advancing. In parallel, cellular reprogramming and organoid engineering are expanding the use of human neuronal models in vitro. This creates an increasing need for tissue culture
Omaditya Khanna et al.
Journal of visualized experiments : JoVE, (66)(66), doi:10-doi:10 (2012-08-22)
Alginate-based materials have received considerable attention for biomedical applications because of their hydrophilic nature, biocompatibility, and physical architecture. Applications include cell encapsulation, drug delivery, stem cell culture, and tissue engineering scaffolds. In fact, clinical trials are currently being performed in
Anna L Hillberg et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 101(2), 258-268 (2012-11-21)
DIABECELL® capsules comprise an inner core of alginate (Alg) coated with a polycationic polymer, poly-L-ornithine (PLO), designed as a stabilizing agent for strengthening the capsule wall, which is masked by an outer layer of biocompatible Alg. These polymeric microcapsules have
Henrik Ring et al.
PloS one, 7(5), e36874-e36874 (2012-05-17)
GABA is more than the main inhibitory neurotransmitter found in the adult CNS. Several studies have shown that GABA regulates the proliferation of progenitor and stem cells. This work examined the effects of the GABA(A) receptor system on the proliferation
Stefano Giovagnoli et al.
Pharmaceutical research, 27(2), 285-295 (2010-01-01)
To assess whether prevention of unexpected in vivo adverse inflammatory and immune responses to biohybrid organ grafts for the treatment of Type I Diabetes Mellitus (T1DM) is possible by superoxide dismutase and ketoprofen controlled release. Superoxide dismutase and ketoprofen-loaded polyester
Nam-Kyung Yu et al.
iScience, 24(11), 103321-103321 (2021-11-25)
A single protein can be multifaceted depending on the cellular contexts and interacting molecules. LIN28A is an RNA-binding protein that governs developmental timing, cellular proliferation, differentiation, stem cell pluripotency, and metabolism. In addition to its best-known roles in microRNA biogenesis
High-throughput, nonperturbing quantification of lipid droplets with digital holographic microscopy.
Vasco Campos et al.
Journal of lipid research, 59(7), 1301-1310 (2018-04-07)
In vitro differentiating adipocytes are sensitive to liquid manipulations and have the tendency to float. Assessing adipocyte differentiation using current microscopy techniques involves cell staining and washing, while using flow cytometry involves cell retrieval in suspension. These methods induce biases
Carl M Sellgren et al.
Nature neuroscience, 22(3), 374-385 (2019-02-06)
Synapse density is reduced in postmortem cortical tissue from schizophrenia patients, which is suggestive of increased synapse elimination. Using a reprogrammed in vitro model of microglia-mediated synapse engulfment, we demonstrate increased synapse elimination in patient-derived neural cultures and isolated synaptosomes.
G Siano et al.
Journal of molecular biology, 432(24), 166675-166675 (2020-10-16)
Neuronal hyperexcitability linked to an increase in glutamate signalling is a peculiar trait of the early stages of Alzheimer's disease (AD) and tauopathies, however, a progressive reduction in glutamate release follows in advanced stages. We recently reported that in the
Annie Kathuria et al.
Genome medicine, 12(1), 34-34 (2020-04-21)
Reprogramming human induced pluripotent stem cells (iPSCs) from somatic cells and generating three-dimensional brain organoids from these iPSCs provide access to live human neuronal tissue with disease-specific genetic backgrounds. Cerebral organoids were generated from iPSCs of eight bipolar disorder (BPI)
Hikari Tanaka et al.
Nature communications, 11(1), 507-507 (2020-01-26)
The timing and characteristics of neuronal death in Alzheimer's disease (AD) remain largely unknown. Here we examine AD mouse models with an original marker, myristoylated alanine-rich C-kinase substrate phosphorylated at serine 46 (pSer46-MARCKS), and reveal an increase of neuronal necrosis
Nicola Micali et al.
Cell reports, 31(5), 107599-107599 (2020-05-07)
Better understanding of the progression of neural stem cells (NSCs) in the developing cerebral cortex is important for modeling neurogenesis and defining the pathogenesis of neuropsychiatric disorders. Here, we use RNA sequencing, cell imaging, and lineage tracing of mouse and
Praneet Opanasopit et al.
International journal of pharmaceutics, 410(1-2), 161-168 (2011-03-15)
The aim of this research was to investigate the effect of cationic polypeptides mixed with chitosan (CS) on in vitro transfection efficiency and cytotoxicity in human cervical carcinoma cells (HeLa cells). The polypeptides/DNA complexes and ternary complexes (CS, polypeptides and
J P McQuilling et al.
Transplantation proceedings, 43(9), 3262-3264 (2011-11-22)
Severe hypoxia caused by a lack of vascular supply and an inability to retrieve encapsulated islets transplanted in the peritoneal cavity for biopsy and subsequent evaluation are obstacles to clinical application of encapsulation strategies for islet transplantation. We recently proposed
Naiyan Wen et al.
International journal of oncology, 55(4), 879-895 (2019-09-06)
Bromodomain and extraterminal domain proteins, especially bromodomain‑containing protein 4 (Brd4), have recently emerged as therapeutic targets for several cancers, although the role and mechanism of Brd4 in glioblastoma multiforme (GBM) are unclear. In this study, we aimed to explore the
Gavin D M Jeffries et al.
Scientific reports, 10(1), 19529-19529 (2020-11-12)
Significant strides have been made in the development of in vitro systems for disease modelling. However, the requirement of microenvironment control has placed limitations on the generation of relevant models. Herein, we present a biological tissue printing approach that employs
Elaine M Harnett et al.
Colloids and surfaces. B, Biointerfaces, 55(1), 90-97 (2007-01-09)
This study calculates the surface energy of polystyrene tissue culture plastic, silicon, silicon dioxide and indium tin oxide, all of which have applications in tissue culture. The adhesion molecules: collagen, fibronectin, poly-L-ornithine and poly-D-lysine, were coated onto these various surfaces
Josine E G Vaes et al.
Glia, 69(3), 655-680 (2020-10-13)
Encephalopathy of prematurity (EoP) is a common cause of long-term neurodevelopmental morbidity in extreme preterm infants. Diffuse white matter injury (dWMI) is currently the most commonly observed form of EoP. Impaired maturation of oligodendrocytes (OLs) is the main underlying pathophysiological
Examination of the biophysical interaction between plasmid DNA and the polycations, polylysine and polyornithine, as a basis for their differential gene transfection in-vitro.
Ramsay E, Hadgraft J, et al.
International Archives of Allergy and Immunology, 210, 97-107 (2000)
QiQi Zhou et al.
Gut, 65(5), 797-805 (2015-02-15)
Many patients with irritable bowel syndrome IBS not only have abdominal pain but also may suffer from visceral hypersensitivity and heighted visceral nociception. Moreover, IBS has few effective therapeutic agents and mechanisms of disease are unclear. Our goals were to
Ke Zhang et al.
Molecular cell, 79(2), 234-250 (2020-06-25)
Somatic cell nuclear transfer (SCNT) can reprogram a somatic nucleus to a totipotent state. However, the re-organization of 3D chromatin structure in this process remains poorly understood. Using low-input Hi-C, we revealed that, during SCNT, the transferred nucleus first enters
Lorraine Iacovitti et al.
Brain research, 1127(1), 19-25 (2006-11-25)
Our ability to use human embryonic stem (hES) cells in cell replacement therapy for Parkinson's disease depends on the discovery of ways to simply and reliably differentiate a dopaminergic (DA) phenotype in these cells. Although several protocols exist for the
Lorenz Uebersax et al.
Biomaterials, 27(26), 4599-4607 (2006-05-20)
Adenosine kinase deficient (Adk-/-) embryonic stem cells (ESCs) encapsulated in synthetic polymers have previously been shown to provide therapeutic adenosine release and transient seizure suppression in epileptic rats. Here we explored the utility of biopolymer-substrates to promote long-term adenosine release
Patricia Martín-Maestro et al.
Frontiers in molecular neuroscience, 10, 291-291 (2017-09-30)
Familial Alzheimer's disease (FAD) is clearly related with the accumulation of amyloid-beta (Aβ) and its deleterious effect on mitochondrial function is well established. Anomalies in autophagy have also been described in these patients. In the present work, functional analyses have
Florian Völlner et al.
Differentiation; research in biological diversity, 77(5), 433-441 (2009-04-28)
Human dental follicle cells (DFCs) derived from wisdom teeth are precursor cells for cementoblasts. In this study, we recognized that naïve DFCs express constitutively the early neural cell marker beta-III-tubulin. Interestingly, DFCs formed beta-III-tubulin-positive neurosphere-like cell clusters (NLCCs) on low-attachment
Efficient DNA transfection of quiescent mammalian cells using poly-L-ornithine.
Y Dong et al.
Nucleic acids research, 21(3), 771-772 (1993-02-11)
Eric J Allenspach et al.
The Journal of experimental medicine, 218(5) (2021-03-17)
SAMD9L is an interferon-induced tumor suppressor implicated in a spectrum of multisystem disorders, including risk for myeloid malignancies and immune deficiency. We identified a heterozygous de novo frameshift variant in SAMD9L in an infant with B cell aplasia and clinical
Michael Peitz et al.
STAR protocols, 1(1), 100038-100038 (2020-10-29)
This protocol describes a highly standardized pipeline for transcription factor-mediated forward programming of human pluripotent stem cells into highly enriched glutamatergic or GABAergic neurons followed by a cryopreservation step that enables the generation of large quality-controlled batches. This approach is particularly
Christopher S Morrow et al.
Cell stem cell, 26(4), 558-568 (2020-02-29)
Maintaining a healthy proteome throughout life is critical for proper somatic stem cell function, but the complexities of the stem cell response to increases in damaged or aggregated proteins remain unclear. Here we demonstrate that adult neural stem cells (NSCs)
Vikram Babu Kasaragod et al.
Neuron, 101(4), 673-689 (2019-02-02)
The frontline anti-malarial drug artemisinin and its derivatives have also been implicated in modulating multiple mammalian cellular pathways, including the recent identification of targeting γ-aminobutyric acid type A receptor (GABAAR) signaling in the pancreas. Their molecular mechanism of action, however
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