P5655

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Enhanced ALP activity of MG63 cells cultured on hydroxyapatite-poly(ethylene glycol) hydrogel composites prepared using EDTA-OH.

Temmei Ito et al.

Biomedical materials (Bristol, England), 10(1), 015025-015025 (2015-03-03)

In order to obtain a hydroxyapatite (HAp)-poly(ethylene glycol) (PEG) composite, tetra amine-terminated PEG was crosslinked using disuccinimidyl tartrate to obtain a PEG hydrogel. Using two kinds of chelators with different stability constants for Ca ion (N-(2-hydroxyethyl) ethylenediamine-N,N',N'-triacetic acid (EDTA-OH, 8.14)

Influence of Different Drying Treatments and Extraction Solvents on the Metabolite Profile and Nitric Oxide Inhibitory Activity of Ajwa Dates.

Nur Ashikin Abdul-Hamid et al.

Journal of food science, 80(11), H2603-H2611 (2015-10-13)

This study aimed to examine the variation in the metabolite profiles and nitric oxide (NO) inhibitory activity of Ajwa dates that were subjected to 2 drying treatments and different extraction solvents. (1)H NMR coupled with multivariate data analysis was employed.

Intracellular distribution of cadmium during the growth of Abortiporus biennis on cadmium-amended media.

Marcin Grąz et al.

Canadian journal of microbiology, 61(8), 545-554 (2015-06-27)

Heavy metals are difficult to remediate and traditional remedial processes are expensive, so bioremediation technology using bacteria, fungi, or plants is of interest. Many studies have demonstrated that basidiomycetes fungi are able to growth under heavy metals stress. In this

Sodium citrate and potassium phosphate as alternative adsorption buffers in hydrophobic and aromatic thiophilic chromatographic purification of plasmid DNA from neutralized lysate.

Nemailla Bonturi et al.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 919-920, 67-74 (2013-02-16)

The number of studies on gene therapy using plasmid vectors (pDNA) has increased in recent years. As a result, the demand for preparations of pDNA in compliance with recommendations of regulatory agencies (EMEA, FDA) has also increased. Plasmid DNA is

Selection and assessment of reference genes for quantitative PCR normalization in migratory locust Locusta migratoria (Orthoptera: Acrididae).

Qingpo Yang et al.

PloS one, 9(6), e98164-e98164 (2014-06-03)

Locusta migratoria is a classic hemimetamorphosis insect and has caused widespread economic damage to crops as a migratory pest. Researches on the expression pattern of functional genes in L. migratoria have drawn focus in recent years, especially with the release

Ultrasound and electric pulses for transdermal drug delivery enhancement: Ex vivo assessment of methods with in vivo oriented experimental protocols.

Barbara Zorec et al.

International journal of pharmaceutics, 490(1-2), 65-73 (2015-05-20)

In our present study we focus on two physical enhancement methods for transdermal drug delivery: ultrasound and electric pulses either alone or in combination. Great emphasis has been given on the design of the experimental system and protocols, so the

Cytosolic, but not matrix, calcium is essential for adjustment of mitochondrial pyruvate supply.

Marten Szibor et al.

The Journal of biological chemistry, 295(14), 4383-4397 (2020-02-26)

Mitochondrial oxidative phosphorylation (OXPHOS) and cellular workload are tightly balanced by the key cellular regulator, calcium (Ca2+). Current models assume that cytosolic Ca2+ regulates workload and that mitochondrial Ca2+ uptake precedes activation of matrix dehydrogenases, thereby matching OXPHOS substrate supply

Effect of telmisartan on VEGF-induced and VEGF-independent angiogenic responsiveness of coronary endothelial cells in normal and streptozotocin (STZ)-induced diabetic rats.

Kiranj K Chaudagar et al.

Clinical and experimental hypertension (New York, N.Y. : 1993), 36(8), 557-566 (2014-02-05)

Telmisartan possesses endothelial protective effects due to angiotensin II type 1 receptor antagonist, peroxisome proliferator-activated receptor γ (PPARγ) agonist and antioxidant action. Therefore, our objective was to study effect of telmisartan on angiogenic responsiveness of coronary endothelial cells (cECs) of

Solid-state properties and dissolution behaviour of tablets containing co-amorphous indomethacin-arginine.

Elisabeth Lenz et al.

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 96, 44-52 (2015-07-22)

Co-amorphous drug formulations provide the possibility to stabilize a drug in its amorphous form by interactions with low molecular weight compounds, e.g. amino acids. Recent studies have shown the feasibility of spray drying as a technique to manufacture co-amorphous indomethacin-arginine

High-level Production of Recombinant Membrane Proteins Using the Engineered Escherichia coli Strains SuptoxD and SuptoxR.

Myrsini Michou et al.

Bio-protocol, 10(15), e3710-e3710 (2021-03-05)

We have previously described the development of two specialized Escherichia coli strains for high-level recombinant membrane protein (MP) production. These engineered strains, termed SuptoxD and SuptoxR, are capable of suppressing the cytotoxicity caused by MP overexpression and of producing greatly

Isoelectric Focusing to Quantify Rhodopsin Phosphorylation in Mouse Retina.

Sowmya Bekshe Lokappa et al.

Bio-protocol, 9(14), e3300-e3300 (2019-07-20)

Rhodopsin is a G-protein coupled receptor (GPCR) that mediates vision under dim light. Upon light exposure, rhodopsin is phosphorylated at multiple serine and threonine sites at its carboxyl-terminus by rhodopsin kinase (GRK1). This, in turn, reduces its ability to activate

RegenHeart: A Time-Effective, Low-Concentration, Detergent-Based Method Aiming for Conservative Decellularization of the Whole Heart Organ.

Eleonora Dal Sasso et al.

ACS biomaterials science & engineering, 6(10), 5493-5506 (2020-12-16)

Heart failure is the worst outcome of all cardiovascular diseases and still represents nowadays the leading cause of mortality with no effective clinical treatments, apart from organ transplantation with allogeneic or artificial substitutes. Although applied as the gold standard, allogeneic

Non-radioactive in vitro PINK1 Kinase Assays Using Ubiquitin or Parkin as Substrate.

Fabienne C Fiesel et al.

Bio-protocol, 6(19) (2017-06-03)

This protocol describes the in vitro phosphorylation of ubiquitin and Parkin by the kinase PINK1 using recombinant proteins. Both substrates, ubiquitin and Parkin, are phosphorylated at the conserved serine 65 residue (pS65-ubiquitin and pS65-Parkin). The protocol also includes the use

The influence of pretreatment methods on saccharification of sugarcane bagasse by an enzyme extract from Chrysoporthe cubensis and commercial cocktails: A comparative study.

Gabriela Piccolo Maitan-Alfenas et al.

Bioresource technology, 192, 670-676 (2015-06-22)

Biomass enzymatic hydrolysis depends on the pretreatment methods employed, the composition of initial feedstock and the enzyme cocktail used to release sugars for subsequent fermentation into ethanol. In this study, sugarcane bagasse was pretreated with 1% H2SO4 and 1% NaOH

Labeling phospholipid membranes with lipid mimetic luminescent metal complexes.

Adam Mechler et al.

Biochimica et biophysica acta, 1838(11), 2939-2946 (2014-08-17)

Lipid-mimetic metallosurfactant based luminophores are promising candidates for labeling phospholipid membranes without altering their biophysical characteristics. The metallosurfactants studied exhibit high structural and physicochemical similarity to phospholipid molecules, designed to incorporate into the membrane structure without the need for covalent

Molecular mechanism of pH-dependent substrate transport by an arginine-agmatine antiporter.

Sheng Wang et al.

Proceedings of the National Academy of Sciences of the United States of America, 111(35), 12734-12739 (2014-08-20)

Enteropathogenic bacteria, exemplified by Escherichia coli, rely on acid-resistance systems (ARs) to survive the acidic environment of the stomach. AR3 consumes intracellular protons through decarboxylation of arginine (Arg) in the cytoplasm and exchange of the reaction product agmatine (Agm) with

Astrocytes in the optic nerve head express putative mechanosensitive channels.

Hee Joo Choi et al.

Molecular vision, 21, 749-766 (2015-08-04)

To establish whether optic nerve head astrocytes express candidate molecules to sense tissue stretch. We used conventional PCR, quantitative PCR, and single-cell reverse transcription PCR (RT-PCR) to assess the expression of various members of the transient receptor potential (TRP) channel

Description of the interaction between Candida albicans and macrophages by mixed and quantitative proteome analysis without isolation.

Nao Kitahara et al.

AMB Express, 5(1), 127-127 (2015-07-17)

Candida albicans is an opportunistic pathogen that causes fatal diseases in immunocompromised hosts. Host resistance against C. albicans relies on ingestion of the pathogen by macrophages. Analysis of the escaping behavior of C. albicans from macrophages is required to understand

Bis-benzimidazole hits against Naegleria fowleri discovered with new high-throughput screens.

Christopher A Rice et al.

Antimicrobial agents and chemotherapy, 59(4), 2037-2044 (2015-01-22)

Naegleria fowleri is a pathogenic free-living amoeba (FLA) that causes an acute fatal disease known as primary amoebic meningoencephalitis (PAM). The major problem for infections with any pathogenic FLA is a lack of effective therapeutics, since PAM has a case

Preparation of Purified Gram-positive Bacterial Cell Wall and Detection in Placenta and Fetal Tissues.

Beth Mann et al.

Bio-protocol, 6(23) (2017-06-03)

Cell wall is a complex biopolymer on the surface of all Gram-positive bacteria. During infection, cell wall is recognized by the innate immune receptor Toll-like receptor 2 causing intense inflammation and tissue damage. In animal models, cell wall traffics from

Effect of ionic liquid pretreatment on the composition, structure and biogas production of water hyacinth (Eichhornia crassipes).

Jing Gao et al.

Bioresource technology, 132, 361-364 (2012-11-29)

The effect of the pretreatment of water hyacinth with ionic liquid and co-solvent on the lignocellulosic composition, structural change and biogas production was evaluated in this study. The results from regenerated water hyacinth indicate that, the content of the lignocellulosic

Butanol production from hexoses and pentoses by fermentation of Clostridium acetobutylicum.

Francesca Raganati et al.

Anaerobe, 34, 146-155 (2015-06-01)

The present paper reports the characterization of ABE (acetone-butanol-ethanol) production by Clostridium acetobutylicum DSM 792 for sugars representative of hydrolysed lignocellulosic biomass (glucose, mannose, arabinose, xylose). The attention was focused on: the selection of an optimal medium for the simultaneous

Outer membrane proteins related to SusC and SusD are not required for Cytophaga hutchinsonii cellulose utilization.

Yongtao Zhu et al.

Applied microbiology and biotechnology, 99(15), 6339-6350 (2015-04-08)

Cytophaga hutchinsonii, a member of the phylum Bacteroidetes, employs a novel collection of cell-associated proteins to digest crystalline cellulose. Other Bacteroidetes rely on cell surface proteins related to the starch utilization system (Sus) proteins SusC and SusD to bind oligosaccharides

Hypoxia and reoxygenation induce endothelial nitric oxide synthase uncoupling in endothelial cells through tetrahydrobiopterin depletion and S-glutathionylation.

Francesco De Pascali et al.

Biochemistry, 53(22), 3679-3688 (2014-04-25)

Ischemia-reperfusion injury is accompanied by endothelial hypoxia and reoxygenation that trigger oxidative stress with enhanced superoxide generation and diminished nitric oxide (NO) production leading to endothelial dysfunction. Oxidative depletion of the endothelial NO synthase (eNOS) cofactor tetrahydrobiopterin can trigger eNOS

Designing greener plasticizers: Effects of alkyl chain length and branching on the biodegradation of maleate based plasticizers.

Hanno C Erythropel et al.

Chemosphere, 134, 106-112 (2015-04-29)

The ubiquitous presence of the plasticizer di (2-ethylhexyl) phthalate (DEHP) in the environment is of concern due to negative biological effects associated with it and its metabolites. In particular, the metabolite mono (2-ethylhexyl) phthalate (MEHP) is a potential endocrine disruptor.

Multi-variable operational characteristic studies of on-column oxidative protein refolding at high loading concentrations.

Pegah Saremirad et al.

Journal of chromatography. A, 1359, 70-75 (2014-08-06)

Chromatographic-based protein refolding techniques have proven to be superior to conventional dilution refolding methods, due to the higher loading concentration and simultaneous purification. Among these techniques, Size Exclusion Chromatography (SEC) has in particular been demonstrated as an effective method for

Ammonium increases Ca(2+) signalling and upregulates expression of Cav1.2 gene in astrocytes in primary cultures and in the in vivo brain.

F Wang et al.

Acta physiologica (Oxford, England), 214(2), 261-274 (2015-04-08)

The primary aim of this study was to identify the effects of hyperammonaemia on functional expression of Cav1.2 L-type Ca(2+) channels in astroglia. Primary cultures of mouse astrocytes were used to study effects of chronic treatment (1-5 days) with ammonium

Assessment of in vitro cyto/genotoxicity of sequentially treated electroplating effluent on the human hepatocarcinoma HuH-7 cell line.

Umesh Chandra Naik et al.

Mutation research. Genetic toxicology and environmental mutagenesis, 762, 9-16 (2014-02-15)

The present study compares in vitro toxicity of electroplating effluent after the batch treatment process with that obtained after the sequential treatment process. Activated charcoal prepared from sugarcane bagasse through chemical carbonization, and tolerant indigenous bacteria, Bacillus sp. strain IST105

Endothelial Progenitor Cells Derived From Wharton's Jelly of Human Umbilical Cord Attenuate Ischemic Acute Kidney Injury by Increasing Vascularization and Decreasing Apoptosis, Inflammation, and Fibrosis.

Chan-Jung Liang et al.

Cell transplantation, 24(7), 1363-1377 (2014-05-14)

Ischemia-reperfusion (I/R) injury to the kidney, a major cause of acute renal failure in humans, is associated with a high mortality, and the development of a new therapeutic strategy is therefore highly desirable. In this study, we examined the therapeutic

Curcumin Attenuates Iron Accumulation and Oxidative Stress in the Liver and Spleen of Chronic Iron-Overloaded Rats.

Farid A Badria et al.

PloS one, 10(7), e0134156-e0134156 (2015-08-01)

Iron overload is now recognized as a health problem in industrialized countries, as excessive iron is highly toxic for liver and spleen. The potential use of curcumin as an iron chelator has not been clearly identified experimentally in iron overload

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