P9416

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Showing 1-30 of 354 results for "P9416" within Papers

Sphingosine kinases and sphingosine-1-phosphate are critical for transforming growth factor beta-induced extracellular signal-regulated kinase 1 and 2 activation and promotion of migration and invasion of esophageal cancer cells.

Anna V Miller et al.

Molecular and cellular biology, 28(12), 4142-4151 (2008-04-23)

Transforming growth factor beta (TGFbeta) plays a dual role in oncogenesis, acting as both a tumor suppressor and a tumor promoter. These disparate processes of suppression and promotion are mediated primarily by Smad and non-Smad signaling, respectively. A central issue

Isolation of functionally distinct mesenchymal stem cell subsets using antibodies against CD56, CD271, and mesenchymal stem cell antigen-1.

Venkata Lokesh Battula et al.

Haematologica, 94(2), 173-184 (2008-12-11)

Conventionally, mesenchymal stem cells are functionally isolated from primary tissue based on their capacity to adhere to a plastic surface. This isolation procedure is hampered by the unpredictable influence of co-cultured hematopoietic and/or other unrelated cells and/or by the elimination

Role of the Plasmodium export element in trafficking parasite proteins to the infected erythrocyte.

Justin A Boddey et al.

Traffic (Copenhagen, Denmark), 10(3), 285-299 (2008-12-06)

The intracellular survival of Plasmodium falciparum within human erythrocytes is dependent on export of parasite proteins that remodel the host cell. Most exported proteins require a conserved motif (RxLxE/Q/D), termed the Plasmodium export element (PEXEL) or vacuolar targeting sequence (VTS)

Genetic dissection of gamma-secretase-dependent and -independent functions of presenilin in regulating neuronal cell cycle and cell death.

Verena Kallhoff-Munoz et al.

The Journal of neuroscience : the official journal of the Society for Neuroscience, 28(44), 11421-11431 (2008-10-31)

Cell cycle markers have been shown to be upregulated and proposed to lead to apoptosis of postmitotic neurons in Alzheimer's disease (AD). Presenilin (PS) plays a critical role in AD pathogenesis, and loss-of-function studies in mice established a potent effect

Identification of human intracellular targets of the medicinal Herb St. John's Wort by chemical-genetic profiling in yeast.

Patrick P McCue et al.

Journal of agricultural and food chemistry, 56(22), 11011-11017 (2008-11-04)

St. John's wort (SJW; Hypericum perforatum L.) is commonly known for its antidepressant properties and was traditionally used to promote wound healing, but its molecular mechanism of action is not known. Here, chemical-genetic profiling in yeast was used to predict

Dynamic Recruitment of Single RNAs to Processing Bodies Depends on RNA Functionality.

Sethuramasundaram Pitchiaya et al.

Molecular cell, 74(3), 521-533 (2019-04-07)

Cellular RNAs often colocalize with cytoplasmic, membrane-less ribonucleoprotein (RNP) granules enriched for RNA-processing enzymes, termed processing bodies (PBs). Here we track the dynamic localization of individual miRNAs, mRNAs, and long non-coding RNAs (lncRNAs) to PBs using intracellular single-molecule fluorescence microscopy.

Translational Regulation of Non-autonomous Mitochondrial Stress Response Promotes Longevity.

Jianfeng Lan et al.

Cell reports, 28(4), 1050-1062 (2019-07-25)

Reduced mRNA translation delays aging, but the underlying mechanisms remain underexplored. Mutations in both DAF-2 (IGF-1 receptor) and RSKS-1 (ribosomal S6 kinase/S6K) cause synergistic lifespan extension in C. elegans. To understand the roles of translational regulation in this process, we performed

Preparation of Onion Epidermal Cell Walls for Imaging by Atomic Force Microscopy (AFM).

Tian Zhang et al.

Bio-protocol, 7(24), e2647-e2647 (2017-12-20)

The growing plant cell wall is comprised of long, thin cellulose microfibrils embedded in a hydrated matrix of polysaccharides and glycoproteins. These components are typically constructed in layers (lamellae) on the inner surface of the cell wall, i.e., between the

Functional characterization of a bioengineered liver after heterotopic implantation in pigs.

Brett D Anderson et al.

Communications biology, 4(1), 1157-1157 (2021-10-09)

Organ bioengineering offers a promising solution to the persistent shortage of donor organs. However, the progression of this technology toward clinical use has been hindered by the challenges of reconstituting a functional vascular network, directing the engraftment of specific functional

Protease-activated receptor 2 is involved in Th2 responses against Trichinella spiralis infection.

Mi Kyung Park et al.

The Korean journal of parasitology, 49(3), 235-243 (2011-11-11)

In order to get a better understanding of the role of protease-activated receptor 2 (PAR2) in type 2 helper T (Th2) cell responses against Trichinella spiralis infection, we analyzed Th2 responses in T. spiralis-infected PAR2 knockout (KO) mice. The levels

Generating primed pluripotent epiblast stem cells: A methodology chapter.

Milan Samanta et al.

Current topics in developmental biology, 138, 139-174 (2020-03-30)

At least two distinct pluripotent states, referred to as naïve and primed, define the early mammalian embryo. In the mouse, the pluripotent epiblast cells in the pre/peri-implantation embryo are the source of naïve embryonic stem cells (ESCs). After the embryo

Lateral flow assay ruler for quantitative and rapid point-of-care testing.

Zhao Li et al.

The Analyst, 144(10), 3314-3322 (2019-04-11)

Lateral flow assay (LFA) is a well-established platform for point-of-care (POC) testing due to its low cost and user friendliness. Conventional LFAs provide qualitative or semi-quantitative results and require dedicated instruments for quantitative detection. Here, we developed an "LFA ruler"

Macrolides Inhibit Capsule Formation of Highly Virulent Cryptococcus gattii and Promote Innate Immune Susceptibility.

Shigeki Nakamura et al.

Antimicrobial agents and chemotherapy, 63(6) (2019-04-03)

Cryptococcus gattii is a fungal pathogen, endemic in tropical and subtropical regions, the west coast of Canada, and the United States, that causes a potentially fatal infection in otherwise healthy individuals. Because the cryptococcal polysaccharide capsule is a leading virulence

Targeting the TXNIP-NLRP3 interaction with PSSM1443 to suppress inflammation in sepsis-induced myocardial dysfunction.

Linhua Wang et al.

Journal of cellular physiology, 236(6), 4625-4639 (2021-01-17)

Sepsis-induced myocardial dysfunction (SIMD), a deadly symptom in sepsis patients, is mainly caused by cardiovascular inflammation. However, it remains unclear how systemic inflammation triggers and aggravates cardiovascular inflammation in the pathogenesis of SIMD. This study found that proinflammatory cytokines and

Direct kinetic fingerprinting and digital counting of single protein molecules.

Tanmay Chatterjee et al.

Proceedings of the National Academy of Sciences of the United States of America, 117(37), 22815-22822 (2020-09-02)

The sensitive and accurate quantification of protein biomarkers plays important roles in clinical diagnostics and biomedical research. Sandwich ELISA and its variants accomplish the capture and detection of a target protein via two antibodies that tightly bind at least two

Retrospective cell lineage reconstruction in humans by using short tandem repeats.

Liming Tao et al.

Cell reports methods, 1(3), None-None (2021-08-04)

Cell lineage analysis aims to uncover the developmental history of an organism back to its cell of origin. Recently, novel in vivo methods utilizing genome editing enabled important insights into the cell lineages of animals. In contrast, human cell lineage remains

TMEM16F phospholipid scramblase mediates trophoblast fusion and placental development.

Yang Zhang et al.

Science advances, 6(19), eaba0310-eaba0310 (2020-06-05)

Cell-cell fusion or syncytialization is fundamental to the reproduction, development, and homeostasis of multicellular organisms. In addition to various cell type-specific fusogenic proteins, cell surface externalization of phosphatidylserine (PS), a universal eat-me signal in apoptotic cells, has been observed in

Metabolic compensation activates pro-survival mTORC1 signaling upon 3-phosphoglycerate dehydrogenase inhibition in osteosarcoma.

Richa Rathore et al.

Cell reports, 34(4), 108678-108678 (2021-01-28)

Osteosarcoma is the most common pediatric and adult primary malignant bone cancer. Curative regimens target the folate pathway, downstream of serine metabolism, with high-dose methotrexate. Here, the rate-limiting enzyme in the biosynthesis of serine from glucose, 3-phosphoglycerate dehydrogenase (PHGDH), is

Polysorbates 20 and 80 used in the formulation of protein biotherapeutics: structure and degradation pathways.

Kerwin BA

Journal of Pharmaceutical Sciences, 97(8), 2924-2935 (2008)

An Ancient Harappan Genome Lacks Ancestry from Steppe Pastoralists or Iranian Farmers.

Vasant Shinde et al.

Cell, 179(3), 729-735 (2019-09-10)

We report an ancient genome from the Indus Valley Civilization (IVC). The individual we sequenced fits as a mixture of people related to ancient Iranians (the largest component) and Southeast Asian hunter-gatherers, a unique profile that matches ancient DNA from

Point mutation in the mouse P2X7 receptor affects intercellular calcium waves in astrocytes.

Sylvia O Suadicani et al.

ASN neuro, 1(1), doi:10-doi:10 (2009-07-03)

Purinergic P2 receptors and gap junctions are two groups of proteins involved in the transmission of ICWs (intercellular calcium waves) between astrocytes. The extent to which ICWs spread among these glial cells depends on the amount of ATP released, which

The Genomic History of the Bronze Age Southern Levant.

Lily Agranat-Tamir et al.

Cell, 181(5), 1146-1157 (2020-05-30)

We report genome-wide DNA data for 73 individuals from five archaeological sites across the Bronze and Iron Ages Southern Levant. These individuals, who share the "Canaanite" material culture, can be modeled as descending from two sources: (1) earlier local Neolithic

Fumarate Upregulates Surface Expression of ULBP2/ULBP5 by Scavenging Glutathione Antioxidant Capacity.

Rikke Illum Høgh et al.

Journal of immunology (Baltimore, Md. : 1950), 204(7), 1746-1759 (2020-03-08)

Fumarate is a tricarboxylic acid cycle metabolite whose intracellular accumulation is linked to inflammatory signaling and development of cancer. In this study, we demonstrate that endogenous fumarate accumulation upregulates surface expression of the immune stimulatory NK group 2, member D

Protein Synthesis in the Developing Neocortex at Near-Atomic Resolution Reveals Ebp1-Mediated Neuronal Proteostasis at the 60S Tunnel Exit.

Matthew L Kraushar et al.

Molecular cell, 81(2), 304-322 (2020-12-29)

Protein synthesis must be finely tuned in the developing nervous system as the final essential step of gene expression. This study investigates the architecture of ribosomes from the neocortex during neurogenesis, revealing Ebp1 as a high-occupancy 60S peptide tunnel exit

Multiplexed Sequential DNA FISH in Caenorhabditis elegans Embryos.

Ahilya N Sawh et al.

STAR protocols, 1(3), 100107-100107 (2020-12-31)

This protocol describes a high-throughput and multiplexed DNA fluorescence in situ hybridization method to trace chromosome conformation in Caenorhabditis elegans embryos. This approach generates single-cell and single-chromosome localization data that can be used to determine chromosome conformation and assess the

Secreted osteopontin is highly polymerized in human airways and fragmented in asthmatic airway secretions.

Mehrdad Arjomandi et al.

PloS one, 6(10), e25678-e25678 (2011-10-28)

Osteopontin (OPN) is a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family and a cytokine with diverse biologic roles. OPN undergoes extensive post-translational modifications, including polymerization and proteolytic fragmentation, which alters its biologic activity. Recent studies suggest that

Optimization of Tetrahydroindazoles as Inhibitors of Human Dihydroorotate Dehydrogenase and Evaluation of Their Activity and In Vitro Metabolic Stability.

Gergana Popova et al.

Journal of medicinal chemistry, 63(8), 3915-3934 (2020-03-28)

Human dihydroorotate dehydrogenase (DHODH), an enzyme in the de novo pyrimidine synthesis pathway, is a target for the treatment of rheumatoid arthritis and multiple sclerosis and is re-emerging as an attractive target for cancer therapy. Here we describe the optimization

Preparation of Cerebellum Granule Neurons from Mouse or Rat Pups and Evaluation of Clostridial Neurotoxin Activity and Their Inhibitors by Western Blot and Immunohistochemistry.

Domenico Azarnia Tehran et al.

Bio-protocol, 8(13), e2918-e2918 (2018-07-05)

Cerebellar Granule Neurons (CGN) from post-natal rodents have been widely used as a model to study neuronal development, physiology and pathology. CGN cultured in vitro maintain the same features displayed in vivo by mature cerebellar granule cells, including the development

Measurement of RNA-induced PKR Activation in vitro.

Kobe C Yuen

Bio-protocol, 7(6), e2178-e2178 (2017-03-20)

Protein kinase R (PKR) is one of the key RNA-activated sensors for innate immunity. PKR is activated by pathogenic or aberrant RNAs such as short double-stranded RNAs or those with imperfect secondary structures, as well as a reduction in the

Pericytes in the myovascular niche promote post-natal myofiber growth and satellite cell quiescence.

Enis Kostallari et al.

Development (Cambridge, England), 142(7), 1242-1253 (2015-03-07)

The satellite cells, which serve as adult muscle stem cells, are both located beneath myofiber basement membranes and closely associated with capillary endothelial cells. We observed that 90% of capillaries were associated with pericytes in adult mouse and human muscle.

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