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pcr cyclers
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Showing 1-30 of 381 results for "pcr cyclers" within Technical Documents
Apoptosis PCR Primers (B8304)
reaction
mixture if not using a thermal cycler with a heated
lid. Place the tube in the thermal cycler when the
thermal cycler reaches 95 oC, and run the
following PCR program.
95 oC for
Data Sheet - F8425
reaction
mixture if not using a thermal cycler with a heated
lid. Place the tube in the thermal cycler when the
thermal cycler reaches 95oC, and run the following
PCR program.
94oC for 2 min
Data Sheet - F8300
mixture if not using a thermal cycler with a heated
lid.
Place the tube in the thermal cycler when the
thermal cycler reaches 95oC, and run the following
PCR program.
94oC for
Product information sheet - XNAS2
Items common to all procedures:
• Tubes or plates for PCR
• PCR primers, Cat. No. OLIGO
• Thermal Cycler
• Water, PCR Reagent, Cat. No. W1754
For individual 1.5
Product Information Sheet - APOPCR
reaction
mixture if not using a thermal cycler with a heated
lid. Place the tube in the thermal cycler when the
thermal cycler reaches 95oC, and run the
following PCR program.
95oC for 2
Product Information Sheet-11636103001
preparing PCR mixes and dilutions
• PCR reaction vessels, such as 0.2 ml thin-walled PCR tubes or plates
• Standard benchtop microcentrifuge
• Thermal block cycler
For standard and hot start PCR
• PCR
Product Information Sheet - D5062
complex PCR targets. Betaine (0.8–1.3 M) has
been reported to improve the amplification of DNA by
reducing secondary structure in GC-rich regions.1
Thermal Cycler
Perkin-Elmer DNA Cyclers 480, 2400
bcl-2 PCR Primers Set
reaction
mixture if not using a thermal cycler with a heated
lid. Place the tube in the thermal cycler when the
thermal cycler reaches 95 oC, and run the following
PCR program.
95 oC for
Product Information Sheet - P4600
or 0.5 ml thin-walled PCR tubes, Catalog
Numbers P3114 and P3364 or plates for specific
thermal cycler
• Thermal cycler
• Mineral Oil, Catalog Number M8662 (optional)
Precautions and Disclaimer
Data Sheet - R4526
Quantitative PCR,
Product Code D 7440 for use with molecular
beacons or dual-labeled probe detection
• Water, PCR reagent, Product Code W 1754
• Primers
• DNA template
• Thermal cycler for quantitative
Product Information Sheet - QR0100
tips
• Plastic PCR tubes, plastic PCR plates, or glass
capillary tubes recommended by the manufacturer
of the spectrofluorometric thermal cycler
• Spectrofluorometric thermal cycler
Precautions
Product Information Sheet-XNAS2
Items common to all procedures:
• Tubes or plates for PCR
• PCR primers, Cat. No. OLIGO
• Thermal Cycler
• Water, PCR Reagent, Cat. No. W1754
For individual 1.5
Product Information Sheet-2gfrmkb
considered fast, while slow cyclers typically have ramp
rates from 1–3°C/sec. The slower the ramp speed, the
shorter the hold time required.
Standard cycling
(fast cyclers)
Ramp rate >3°C/sec
Product information sheet -XNAS
Provided)
Items common to all procedures:
• Tubes or plates for PCR
• PCR primers, Cat. No. OLIGO
• Thermal cycler
• Water, PCR grade, Cat. No. W1754
For individual
Product Information Sheet-XNAS
Provided)
Items common to all procedures:
• Tubes or plates for PCR
• PCR primers, Cat. No. OLIGO
• Thermal cycler
• Water, PCR grade, Cat. No. W1754
For individual
Product Information Sheet-thificdnaro
two-step RT-PCR, using
qualitative RT-PCR on conventional thermal cyclers or quantitative RT-PCR on the LightCycler® Carousel-Based
System, the LightCycler® 480 System, or other real-time PCR instruments
Product Information Sheet - D1816
required but not provided
• Template to be amplified
• Primers
• Thermal cycler
• Dedicated pipettes
• PCR pipette tips
• 0.2 or 0.5 ml PCR microcentrifuge tubes,
thin-walled, Catalog Numbers P3114 and P3364
Product Information Sheet - D5813
prevent evaporation if using a thermal cycler
without a heated lid.
4. The amplification parameters will vary depending
on the primers and the thermal cycler used. It may
be necessary to optimize
Product Information Sheet-dutp-ro
PCR
The following thermal profile is an example. Different thermal cyclers may require different profiles.
Overlay the reaction carefully with mineral oil if required by the thermal cycler.
Product Information Sheet - D7440
plates for specific thermal cycler
• Thermal cycler for quantitative PCR
Precautions and Disclaimer
This product is for R&D use only, not for drug,
household, or other uses. Please consult the Material
Product Information Sheet - D6063
prevent evaporation if using a thermal cycler
without a heated lid.
4. The amplification parameters will vary depending
on the primers and the thermal cycler used. It may
be necessary to optimize
Product Information Sheet - D1691
Required But Not
Provided
• Template to be amplified
• Primers
• Thermal cycler
• Dedicated pipettes
• PCR pipette tips
• 0.2 or 0.5 ml PCR microcentrifuge tubes, thin-
walled, Catalog Numbers P3114 and P3364
Product Information Sheet - D4545
D7295
containing 10 mM dATP, dCTP, dGTP, TTP
• Water, PCR Reagent, Catalog No. W1754
• Mineral Oil, Catalog No. M8662 (optional)
• Thermal cycler
• Primers
• DNA to be amplified
Precautions and Disclaimer
Product Information Sheet - D4545
D7295
containing 10 mM dATP, dCTP, dGTP, TTP
• Water, PCR Reagent, Catalog No. W1754
• Mineral Oil, Catalog No. M8662 (optional)
• Thermal cycler
• Primers
• DNA to be amplified
Precautions and Disclaimer
Product Information Sheet-pfabikb
be 100 – 500 nM.
ROX Reference Dye
For certain real-time cyclers, the presence of the passive
reference dye, ROX, compensates for non-PCR related
variations in fluorescence detection. Fluorescence
Specification Sheet
skirted and full-skirted PCR plates as well as deep-
well plates
NOT recommended for qPCR
Bundling Opportunities
PCR plates
PCR reagents
Thermal cyclers
Product information sheet - XNATP
WHAWB100014
• Tubes or plate for PCR
• Heat block or thermal cycler at 95 °C
• PCR Primers (Cat. No. OLIGO)
• Thermal cycler
Product information sheet - XNAT2
WHAWB100014)
• Tubes or plate for PCR
• Heat block or thermal cycler at 95 °C
• PCR Primers (Cat. No. OLIGO)
• Thermal cycler
Product Information Sheet-XNAT2
WHAWB100014)
• Tubes or plate for PCR
• Heat block or thermal cycler at 95 °C
• PCR Primers (Cat. No. OLIGO)
• Thermal cycler
Product Information Sheet - D8045
Perkin-Elmer DNA Cycler 480 has been used to
develop cycling parameters. Other types of thermal
cyclers can also be used, but may require further
optimization of cycling parameters.
Primer design
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