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Showing 1-30 of 120 results for "PKH67GL" within Papers
Estefanía García-Guerrero et al.
Frontiers in immunology, 9, 1971-1971 (2018-09-21)
The relevance of the immune system in cancer has long been studied. Autologous adoptive T cell therapies, based on the use of tumor infiltrating lymphocytes (TILs), have made great progress in recent years for the treatment of solid tumors, especially
Anjali P Kusumbe et al.
Cancer research, 69(24), 9245-9253 (2009-12-03)
Tumor formation involves substantial cell division and genetic instability, but the relationship between quiescent cancer stem cells (CSC) and dividing progenitors in these events is poorly understood. Likewise, the implication of aneuploid cells in solid tumors is uncertain. CSCs are
Wooseok Seo et al.
Nature communications, 11(1), 1562-1562 (2020-03-29)
CCL5 is a unique chemokine with distinct stage and cell-type specificities for regulating inflammation, but how these specificities are achieved and how CCL5 modulates immune responses is not well understood. Here we identify two stage-specific enhancers: the proximal enhancer mediates
Christian Schütz et al.
Journal of immunological methods, 344(2), 98-108 (2009-04-01)
Novel immunosuppressive strategies are targeting for an antigen-specific deletion of T cells responsible for organ damage in autoimmunity and allograft rejection. Here, we present a new flow cytometry-based assay that allows the reliable and efficient detection of T cells that
Shunsuke Eguchi et al.
The Journal of biological chemistry, 294(31), 11665-11674 (2019-06-21)
Extracellular vesicles (EVs) have emerged as key mediators of intercellular communication that have the potential to improve cardiac function when used in cell-based therapy. However, the means by which cardiomyocytes respond to EVs remains unclear. Here, we sought to clarify
William E Ruff et al.
Cell host & microbe, 26(1), 100-113 (2019-06-23)
Given the immense antigenic load present in the microbiome, we hypothesized that microbiota mimotopes can be a persistent trigger in human autoimmunity via cross-reactivity. Using antiphospholipid syndrome (APS) as a model, we demonstrate cross-reactivity between non-orthologous mimotopes expressed by a common
Regina Gary et al.
Journal of immunology (Baltimore, Md. : 1950), 188(2), 744-752 (2011-12-17)
Upon specific interaction with APCs, T cells capture membrane fragments and surface molecules in a process termed trogocytosis. In this study, we demonstrate that human Ag-specific CD8(+) T cells acquire the coinhibitory molecule programmed death ligand 1 (PD-L1) from mature
Vartika Sharma et al.
Autophagy, 17(2), 476-495 (2020-02-23)
Opportunistic bacterial infections amongst HIV-infected individuals contribute significantly to HIV-associated mortality. The role of HIV-mediated modulation of innate mechanisms like autophagy in promoting opportunistic infections, however, remains obscure. Here we show, HIV reactivation in or infection of macrophages inhibits autophagy
Chia-Hao Wu et al.
The Journal of biological chemistry, 294(9), 3207-3218 (2018-12-30)
The field cancerization effect has been proposed to explain bladder cancer's multifocal and recurrent nature, yet the mechanisms of this effect remain unknown. In this work, using cell biology, flow cytometry, and qPCR analyses, along with a xenograft mouse tumor
Yu Jun Tan et al.
Scientific reports, 6, 39140-39140 (2016-12-15)
A hybrid 3D bioprinting approach using porous microscaffolds and extrusion-based printing method is presented. Bioink constitutes of cell-laden poly(D,L-lactic-co-glycolic acid) (PLGA) porous microspheres with thin encapsulation of agarose-collagen composite hydrogel (AC hydrogel). Highly porous microspheres enable cells to adhere and
Xiaoxuan Zhuang et al.
Frontiers in immunology, 10, 2879-2879 (2020-01-11)
The anti-leukemia activity of NK cells helps prevent relapse during hematopoietic stem cell transplantation (HSCT) in leukemia patients. However, the factors that determine the sensitivity or resistance of leukemia cells in the context of NK-mediated cytotoxicity are not well-established. Here
Paul K Wallace et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 73(11), 1019-1034 (2008-09-13)
Cell-tracking reagents such as the green-fluorescent protein labeling dye CFSE and the red-fluorescent lipophilic membrane dye PKH26 are commonly used to monitor cell proliferation by flow cytometry in heterogeneous cell populations responding to immune stimuli. Both reagents stain cells with
Abdelnaby Khalyfa et al.
Frontiers in neurology, 8, 709-709 (2018-01-10)
Intermittent hypoxia (IH) is associated with increased endothelial dysfunction and cardiovascular disorders. Exosomes released in biological fluids may act as vehicles for propagating such damage, modifying the functional phenotype of endothelial cells. Drug interventions, however, may provide protection for the
Wan-Ning Li et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(41), 25859-25868 (2020-10-03)
Endometriosis is a highly prevalent gynecological disease with severe negative impacts on life quality and financial burden. Unfortunately, there is no cure for this disease, which highlights the need for further investigation about the pathophysiology of this disease to provide
Erez Eitan et al.
Journal of extracellular vesicles, 4, 26373-26373 (2015-03-31)
Fetal bovine serum (FBS) is the most widely used serum supplement for mammalian cell culture. It supports cell growth by providing nutrients, growth signals, and protection from stress. Attempts to develop serum-free media that support cell expansion to the same
Wei Sun et al.
Frontiers in bioengineering and biotechnology, 8, 584410-584410 (2020-11-17)
Promoting cell spreading is crucial to enhance bone healing and implant osteointegration. In this study, we investigated the stimulatory effect of human salivary histatin-1 (Hst-1) on the spreading of osteogenic cells in vitro as well as the potential signaling pathways
Kyoung Hoon Lee et al.
mAbs, 10(3), 380-396 (2018-02-23)
CT-P10 (Truxima™) was recently approved as the world's first rituximab biosimilar product in the European Union (EU) and South Korea. To demonstrate biosimilarity of CT-P10 with the reference medicinal product (RMP), extensive 3-way similarity assessment has been conducted between CT-P10
Shirong Cao et al.
Nature communications, 13(1), 4684-4684 (2022-08-11)
Obesity and obesity-related health complications are increasing in prevalence. Adipose tissue from obese subjects has low-grade, chronic inflammation, leading to insulin resistance. Adipose tissue macrophages (ATMs) are a source of proinflammatory cytokines that further aggravate adipocyte dysfunction. In response to
Yihua Bei et al.
Basic research in cardiology, 112(4), 38-38 (2017-05-24)
Extracellular vesicles (EVs) serve an important function as mediators of intercellular communication. Exercise is protective for the heart, although the signaling mechanisms that mediate this cardioprotection have not been fully elucidated. Here using nano-flow cytometry, we found a rapid increase
Hong Wei et al.
Cell death & disease, 11(4), 290-290 (2020-04-29)
Hypoxia limits the survival and function of neurons in the development of Alzheimer's diseases. Exosome-dependent intercellular communication is an emerging signaling mechanism involved in tissue repair and regeneration; however, the effect and underlying mechanism of mesenchymal stem cell-derived exosomes in
Kyoung Jin Lee et al.
PloS one, 15(7), e0235356-e0235356 (2020-07-07)
As a new class of cancer therapeutic agents, oncolytic viruses (OVs) have gained much attention not only due to their ability to selectively replicate in and lyse tumor cells, but also for their potential to stimulate antitumor immune responses. As
Nobuyoshi Kosaka et al.
The Journal of biological chemistry, 287(2), 1397-1405 (2011-11-30)
Normal epithelial cells regulate the secretion of autocrine and paracrine factors that prevent aberrant growth of neighboring cells, leading to healthy development and normal metabolism. One reason for tumor initiation is considered to be a failure of this homeostatic cell
Alexandre Chigaev et al.
BMC immunology, 9, 26-26 (2008-06-07)
Activation of integrins in response to inside-out signaling serves as a basis for leukocyte arrest on endothelium, and migration of immune cells. Integrin-dependent adhesion is controlled by the conformational state of the molecule (i.e. change in the affinity for the
Ying Xiang et al.
Cell death & disease, 13(7), 599-599 (2022-07-13)
The emerging roles of extracellular vesicles (EVs) in bladder cancer have recently been identified. This study aims to elucidate the role of microRNA-139-5p (miR-139-5p) shuttled by bone marrow mesenchymal stem cells (BMSCs)-derived EVs (BMSCs-EVs) in bladder cancer, with the possible
Xiao-Jing Ye et al.
Anti-cancer agents in medicinal chemistry, 21(6), 766-774 (2020-08-05)
Our previous work demonstrated upregulated CD47 in Oral Squamous Cell Carcinoma (OSCC). In the present study, we aimed to investigate the effects of CD47 on tumor cell development and phagocytosis in OSCC and elucidate the underlying mechanisms. The proliferation, apoptosis
Transcriptome Reprogramming of CD11b+ Bone Marrow Cells by Pancreatic Cancer Extracellular Vesicles.
Joana Maia et al.
Frontiers in cell and developmental biology, 8, 592518-592518 (2020-12-18)
Pancreatic cancers (PC) are highly metastatic with poor prognosis, mainly due to delayed detection. We previously showed that PC-derived extracellular vesicles (EVs) act on macrophages residing in the liver, eliciting extracellular matrix remodeling in this organ and marked hepatic accumulation
Ting Huyan et al.
Iranian journal of public health, 47(6), 803-813 (2018-08-09)
Cancer is the leading cause of death in human disease and is a major public health problem around the world. Exosomes are a promising cancer biomarker and therapy target. Recent evidence demonstrate that tumor cells could inhibit natural killer (NK)
Alexandre Chigaev et al.
BMC immunology, 15, 52-52 (2014-11-05)
Carbon monoxide (CO), a byproduct of heme degradation, is attracting growing attention from the scientific community. At physiological concentrations, CO plays a role as a signal messenger that regulates a number of physiological processes. CO releasing molecules are under evaluation
Susan J Zunino et al.
The Journal of nutrition, 139(8), 1603-1608 (2009-06-25)
We hypothesized that the phytochemicals resveratrol, quercetin, and kaempferol would modulate B lymphocyte proliferation, Ig synthesis, and apoptosis after activation. Peripheral blood mononuclear cells (PBMC) were isolated from 12 healthy adult human volunteers and incubated with pokeweed mitogen plus 0
Amit Singh et al.
JCI insight, 4(13) (2019-07-12)
Bone provides supportive microenvironments for hematopoietic stem cells (HSCs) and mesenchymal stem cells (MSCs) and is a frequent site of metastasis. While incidences of bone metastases increase with age, the properties of the bone marrow microenvironment that regulate dormancy and
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