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Showing 1-30 of 120 results for "PKH67GL" within Papers
Janine C Deddens et al.
Journal of cardiovascular translational research, 9(4), 291-301 (2016-07-08)
Plasma-circulating microRNAs have been implicated as novel early biomarkers for myocardial infarction (MI) due to their high specificity for cardiac injury. For swift clinical translation of this potential biomarker, it is important to understand their temporal and spatial characteristics upon
Sergio Ramirez et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 53(1), 55-65 (2003-04-18)
We recently reported an automated sample handling system, designated HyperCyt, by which samples are aspirated from microplate wells and delivered to the flow cytometer for analysis at rates approaching 100 samples per minute. In this approach, an autosampler and peristaltic
Y Wang et al.
Oncogene, 30(12), 1470-1480 (2010-11-26)
Recent studies indicate that a subset of cancer cells possessing stem cell properties, referred to as cancer-initiating or cancer stem cells (CSCs), have crucial roles in tumor initiation, metastasis and resistance to anticancer therapies. Transforming growth factor (TGF)-β and their
Van Sang Nguyen et al.
Structure (London, England : 1993), 23(11), 2022-2031 (2015-10-07)
Type III secretion systems (T3SSs) are adopted by pathogenic bacteria for the transport of effector proteins into host cells through the translocon pore composed of major and minor translocator proteins. Both translocators require a dedicated chaperone for solubility. Despite tremendous
Yu Suk Choi et al.
Biomaterials, 33(8), 2482-2491 (2011-12-27)
Though reduced serum or myoblast co-culture alone can differentiate adipose-derived stem cells (ASCs) into mesenchymal lineages, efficiency is usually not sufficient to restore function in vivo. Often when injected into fibrotic muscle, their differentiation may be misdirected by the now stiffened
Liwen Luo et al.
Bioactive materials, 15, 29-43 (2022-04-08)
Low back pain, mainly caused by intervertebral disc degeneration (IVDD), is a common health problem; however, current surgical treatments are less than satisfactory. Thus, it is essential to develop novel non-invasive surgical methods for IVDD treatment. Here, we describe a
Xiao-Hong Wang et al.
Transplant international : official journal of the European Society for Organ Transplantation, 25(1), 118-126 (2011-10-25)
Activation of NF-κB pathway and co-stimulatory system CD40/CD40L promotes the inflammation, which plays a key role in the failure of islet graft. Therefore, the purpose of this study was to determine if simultaneous blockade of CD40/CD40L and IκB/NF-κB pathways could
Arianna Venturini et al.
Frontiers in pharmacology, 10, 1452-1452 (2019-12-19)
It is widely recognized that extracellular vesicles subserve non-classical signal transmission in the central nervous system. Here we assess if the astrocyte processes, that are recognized to play crucial roles in intercellular communication at the synapses and in neuron-astrocyte networks
Sun Ah Jeong et al.
Expert opinion on biological therapy, 19(10), 1085-1095 (2018-12-14)
Objectives: Therapeutic monoclonal antibody biosimilars are expected to help reduce the sizeable economic burden of targeted treatments. Trastuzumab (Herceptin®), a recombinant humanized monoclonal antibody that binds to the extracellular domain of HER2, is approved for use in HER2-overexpressing breast cancer
Shunsuke Eguchi et al.
The Journal of biological chemistry, 294(31), 11665-11674 (2019-06-21)
Extracellular vesicles (EVs) have emerged as key mediators of intercellular communication that have the potential to improve cardiac function when used in cell-based therapy. However, the means by which cardiomyocytes respond to EVs remains unclear. Here, we sought to clarify
William E Ruff et al.
Cell host & microbe, 26(1), 100-113 (2019-06-23)
Given the immense antigenic load present in the microbiome, we hypothesized that microbiota mimotopes can be a persistent trigger in human autoimmunity via cross-reactivity. Using antiphospholipid syndrome (APS) as a model, we demonstrate cross-reactivity between non-orthologous mimotopes expressed by a common
Vartika Sharma et al.
Autophagy, 17(2), 476-495 (2020-02-23)
Opportunistic bacterial infections amongst HIV-infected individuals contribute significantly to HIV-associated mortality. The role of HIV-mediated modulation of innate mechanisms like autophagy in promoting opportunistic infections, however, remains obscure. Here we show, HIV reactivation in or infection of macrophages inhibits autophagy
Abdelnaby Khalyfa et al.
Frontiers in neurology, 8, 709-709 (2018-01-10)
Intermittent hypoxia (IH) is associated with increased endothelial dysfunction and cardiovascular disorders. Exosomes released in biological fluids may act as vehicles for propagating such damage, modifying the functional phenotype of endothelial cells. Drug interventions, however, may provide protection for the
Valeria V Orlova et al.
Arteriosclerosis, thrombosis, and vascular biology, 34(1), 177-186 (2013-10-26)
Endothelial cells (ECs), pericytes, and vascular smooth muscle cells (vSMCs) are essential for vascular development, and their dysfunction causes multiple cardiovascular diseases. Primary vascular cells for research are, however, difficult to obtain. Human-induced pluripotent stem cells (hiPSCs) derived from somatic
Han Lu et al.
Autophagy, 17(9), 2511-2527 (2020-10-09)
Deficiency in decidualization has been widely regarded as an important cause of spontaneous abortion. Generalized decidualization also includes massive infiltration and enrichment of NK cells. However, the underlying mechanism of decidual NK (dNK) cell residence remains largely unknown. Here, we
Susan J Zunino et al.
The Journal of nutrition, 139(8), 1603-1608 (2009-06-25)
We hypothesized that the phytochemicals resveratrol, quercetin, and kaempferol would modulate B lymphocyte proliferation, Ig synthesis, and apoptosis after activation. Peripheral blood mononuclear cells (PBMC) were isolated from 12 healthy adult human volunteers and incubated with pokeweed mitogen plus 0
Christian Schütz et al.
Journal of immunological methods, 344(2), 98-108 (2009-04-01)
Novel immunosuppressive strategies are targeting for an antigen-specific deletion of T cells responsible for organ damage in autoimmunity and allograft rejection. Here, we present a new flow cytometry-based assay that allows the reliable and efficient detection of T cells that
Amit Singh et al.
JCI insight, 4(13) (2019-07-12)
Bone provides supportive microenvironments for hematopoietic stem cells (HSCs) and mesenchymal stem cells (MSCs) and is a frequent site of metastasis. While incidences of bone metastases increase with age, the properties of the bone marrow microenvironment that regulate dormancy and
Alexandre Chigaev et al.
BMC immunology, 15, 52-52 (2014-11-05)
Carbon monoxide (CO), a byproduct of heme degradation, is attracting growing attention from the scientific community. At physiological concentrations, CO plays a role as a signal messenger that regulates a number of physiological processes. CO releasing molecules are under evaluation
Wei Sun et al.
Frontiers in bioengineering and biotechnology, 8, 584410-584410 (2020-11-17)
Promoting cell spreading is crucial to enhance bone healing and implant osteointegration. In this study, we investigated the stimulatory effect of human salivary histatin-1 (Hst-1) on the spreading of osteogenic cells in vitro as well as the potential signaling pathways
Yu Jun Tan et al.
Scientific reports, 6, 39140-39140 (2016-12-15)
A hybrid 3D bioprinting approach using porous microscaffolds and extrusion-based printing method is presented. Bioink constitutes of cell-laden poly(D,L-lactic-co-glycolic acid) (PLGA) porous microspheres with thin encapsulation of agarose-collagen composite hydrogel (AC hydrogel). Highly porous microspheres enable cells to adhere and
Xiaoxuan Zhuang et al.
Frontiers in immunology, 10, 2879-2879 (2020-01-11)
The anti-leukemia activity of NK cells helps prevent relapse during hematopoietic stem cell transplantation (HSCT) in leukemia patients. However, the factors that determine the sensitivity or resistance of leukemia cells in the context of NK-mediated cytotoxicity are not well-established. Here
Chia-Hao Wu et al.
The Journal of biological chemistry, 294(9), 3207-3218 (2018-12-30)
The field cancerization effect has been proposed to explain bladder cancer's multifocal and recurrent nature, yet the mechanisms of this effect remain unknown. In this work, using cell biology, flow cytometry, and qPCR analyses, along with a xenograft mouse tumor
Regina Gary et al.
Journal of immunology (Baltimore, Md. : 1950), 188(2), 744-752 (2011-12-17)
Upon specific interaction with APCs, T cells capture membrane fragments and surface molecules in a process termed trogocytosis. In this study, we demonstrate that human Ag-specific CD8(+) T cells acquire the coinhibitory molecule programmed death ligand 1 (PD-L1) from mature
Paul K Wallace et al.
Cytometry. Part A : the journal of the International Society for Analytical Cytology, 73(11), 1019-1034 (2008-09-13)
Cell-tracking reagents such as the green-fluorescent protein labeling dye CFSE and the red-fluorescent lipophilic membrane dye PKH26 are commonly used to monitor cell proliferation by flow cytometry in heterogeneous cell populations responding to immune stimuli. Both reagents stain cells with
Kyoung Hoon Lee et al.
mAbs, 10(3), 380-396 (2018-02-23)
CT-P10 (Truxima™) was recently approved as the world's first rituximab biosimilar product in the European Union (EU) and South Korea. To demonstrate biosimilarity of CT-P10 with the reference medicinal product (RMP), extensive 3-way similarity assessment has been conducted between CT-P10
Wan-Ning Li et al.
Proceedings of the National Academy of Sciences of the United States of America, 117(41), 25859-25868 (2020-10-03)
Endometriosis is a highly prevalent gynecological disease with severe negative impacts on life quality and financial burden. Unfortunately, there is no cure for this disease, which highlights the need for further investigation about the pathophysiology of this disease to provide
Transcriptome Reprogramming of CD11b+ Bone Marrow Cells by Pancreatic Cancer Extracellular Vesicles.
Joana Maia et al.
Frontiers in cell and developmental biology, 8, 592518-592518 (2020-12-18)
Pancreatic cancers (PC) are highly metastatic with poor prognosis, mainly due to delayed detection. We previously showed that PC-derived extracellular vesicles (EVs) act on macrophages residing in the liver, eliciting extracellular matrix remodeling in this organ and marked hepatic accumulation
Ting Huyan et al.
Iranian journal of public health, 47(6), 803-813 (2018-08-09)
Cancer is the leading cause of death in human disease and is a major public health problem around the world. Exosomes are a promising cancer biomarker and therapy target. Recent evidence demonstrate that tumor cells could inhibit natural killer (NK)
Erez Eitan et al.
Journal of extracellular vesicles, 4, 26373-26373 (2015-03-31)
Fetal bovine serum (FBS) is the most widely used serum supplement for mammalian cell culture. It supports cell growth by providing nutrients, growth signals, and protection from stress. Attempts to develop serum-free media that support cell expansion to the same
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