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Showing 1-30 of 362 results for "S8282" within Papers
Zhengming Gu et al.
Archives of pathology & laboratory medicine, 138(11), 1474-1480 (2014-10-31)
Invasive fungal infections are an important cause of morbidity and mortality among immunocompromised patients. To design and evaluate a multiplexed assay aimed at quantitative detection and differentiation of the 5 molds that are most commonly responsible for pulmonary infections. Using
R Kanďár et al.
Andrologia, 46(10), 1079-1088 (2013-10-16)
A method is described here for the determination of total glutathione (TGSH) and glutathione disulphide (GSSG) in the seminal plasma of the male partners of couples requesting a fertility evaluation. A suitable sample preparation procedure prior to high-performance liquid chromatography
Xu Wang et al.
The Analyst, 140(5), 1453-1458 (2015-01-21)
A simple, sensitive and cost-effective method for the analysis of the mycotoxin aflatoxin B1 (AFB1) has been established based on controlled growth of immunogold. AFB1-BSA conjugate modified magnetic beads were employed as capture probe and anti-AFB1 antibody-coated gold colloids were
Florencia Fernandez-Chiappe et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 41(4), 689-710 (2020-12-03)
Circadian rhythms have been extensively studied in Drosophila; however, still little is known about how the electrical properties of clock neurons are specified. We have performed a behavioral genetic screen through the downregulation of candidate ion channels in the lateral
Ngoc Tung Tran et al.
STAR protocols, 1(1), 100028-100028 (2020-07-21)
Mutations that accumulate in self-renewing hematopoietic stem and progenitor cells (HSPCs) can cause severe blood disorders. To model such disorders in mice, we developed a CRISPR/Cas9/adeno-associated virus (AAV)-based system to knock in and repair genes by homologous recombination in mouse
Emil Meng-Lund et al.
Journal of pharmaceutical sciences, 103(7), 2053-2061 (2014-05-16)
The pH partition theory proposes a correlation between fraction of unionized drug substance and permeability. The aim of this study was to compare the permeability of metoprolol and mannitol in ex vivo human and porcine buccal mucosa models at varying
Vibhav Gautam et al.
3 Biotech, 9(5), 193-193 (2019-05-09)
The functional characterization of miRNAs often involves understanding of their spatiotemporal expression, which mostly relies on reporter-based or in situ hybridization studies. The available in situ localization methods follow separate protocols for pre-hybridization, hybridization, post-hybridization, and detection steps for both miRNA and mRNA transcripts
Wim Van Gansbeke et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1001, 221-240 (2015-08-22)
In 2013, the World Anti-Doping Agency (WADA) drastically lowered the minimum required performance levels (MRPLs) of most doping substances, demanding a substantial increase in sensitivity of the existing methods. For a number of compounds, conventional electron impact ionization gas chromatography
Colonoscopy preperations.
The Medical letter on drugs and therapeutics, 55(1408), 6-6 (2013-01-26)
Ewelina Mroczek et al.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment, 32(12), 2113-2119 (2015-09-29)
The paper investigates the migration of the acrylamide monomer (AMD) to lettuce chosen as a test plant growing in an organic medium (peat substrate). Polyacrylamide (PAM)-based flocculant added to the growing medium contained no more than 1000 mg kg(-1) of
Yiwen Li et al.
Nature protocols, 3(11), 1703-1708 (2008-10-11)
We describe a protocol to rapidly and reliably visualize blood vessels in experimental animals. Blood vessels are directly labeled by cardiac perfusion using a specially formulated aqueous solution containing 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI), a lipophilic carbocyanine dye, which incorporates into endothelial
Franziska E Uhl et al.
The European respiratory journal, 46(4), 1150-1166 (2015-05-02)
Chronic obstructive pulmonary disease (COPD) is characterised by a progressive loss of lung tissue. Inducing repair processes within the adult diseased lung is of major interest and Wnt/β-catenin signalling represents a promising target for lung repair. However, the translation of
Maria Cristina Straccia et al.
Marine drugs, 13(5), 2890-2908 (2015-05-15)
In this work, a coating of chitosan onto alginate hydrogels was realized using the water-soluble hydrochloride form of chitosan (CH-Cl), with the dual purpose of imparting antibacterial activity and delaying the release of hydrophilic molecules from the alginate matrix. Alginate
Alexander M Walter et al.
eLife, 6 (2017-10-27)
Phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] is essential for exocytosis. Classical ways of manipulating PI(4,5)P2 levels are slower than its metabolism, making it difficult to distinguish effects of PI(4,5)P2 from those of its metabolites. We developed a membrane-permeant, photoactivatable PI(4,5)P2, which is loaded into
Andrea Cossu et al.
International journal of pharmaceutics, 493(1-2), 233-242 (2015-08-02)
Conventional antifungal treatments against Candida albicans in the oral cavity often result in increased cytotoxicity. The goal of this study was to determine the potential of starch Pickering emulsion as a delivery vehicle for an antifungal natural phenolic compound such
Raquel dos Santos et al.
Journal of chromatography. A, 1355, 115-124 (2014-06-21)
In this work, phenylboronic acid (PBA) was thoroughly investigated as a synthetic ligand for the purification of an immunoglobulin G (IgG) from a clarified cell supernatant from Chinese Hamster Ovary (CHO) cell cultures. In particular, the study was focused on
Kishore Cholkar et al.
AAPS PharmSciTech, 15(6), 1454-1467 (2014-07-02)
The purpose of this study was to develop a clear aqueous mixed nanomicellar formulation (MNF) of dexamethasone utilizing both D-α-tocopherol polyethylene glycol-1000 succinate (Vit E TPGS) and octoxynol-40 (Oc-40). In this study, Vit E TPGS and Oc-40 are independent variables.
Jiri Zeravik et al.
Food chemistry, 194, 432-440 (2015-10-17)
Biosensors based on lactate oxidase, sarcosine oxidase and mixture of fumarase and sarcosine oxidase were used for monitoring of organic acids in wine samples. Additionally, tartaric acid was determined by modified colorimetric method based on formation of the vanadate-tartrate complex.
J Rodenas-Montano et al.
Journal of chromatography. A, 1344, 31-41 (2014-04-29)
In micellar liquid chromatography (MLC), chromatographic peaks are more evenly distributed compared to conventional reversed-phase liquid chromatography (RPLC). This is the reason that most procedures are implemented using isocratic elution. However, gradient elution may be still useful in MLC to
Hossein Abdolmohammad-Zadeh et al.
Analytica chimica acta, 881, 54-64 (2015-06-05)
A simple dispersive solid-phase micro-extraction method based on CoFe2O4 nano-particles (NPs) functionalized with 8-hydroxyquinoline (8-HQ) with the aid of sodium dodecyl sulfate (SDS) was developed for separation of Al(III) ions from aqueous solutions. Al(III) ions are separated at pH 7
L Boysen et al.
Poultry science, 92(5), 1425-1429 (2013-04-11)
When assessing effects of decontamination techniques on counts of Campylobacter spp. on broiler meat, it is essential that the results reflect the variations that may exist. Decontamination studies often use high inoculation levels (10(7) to 10(8) cfu) and one or
Anne-Lise Marie et al.
Journal of pharmaceutical and biomedical analysis, 111, 64-70 (2015-04-12)
With the aim to determine the binding affinity of a new generation of recombinant antithrombin (AT) toward heparin, we developed a dynamic equilibrium-affinity capillary electrophoresis (DE-ACE) method. This method allows the determination of an AT-heparin binding constant (Kd) directly from
Asdren Zajmi et al.
PloS one, 10(6), e0128157-e0128157 (2015-06-02)
Staphylococci are facultative anaerobes, perfectly spherical un-encapsulated cocci, with a diameter not exceeding 1 micrometer in diameter. Staphylococcus aureus are generally harmless and remain confined to the skin unless they burrow deep into the body, causing life-threatening infections in bones
Yang-Chi Fan et al.
Journal of AOAC International, 98(3), 575-579 (2015-05-31)
Danofloxacin is an antibacterial drug of the fluoroquinolone group developed for therapeutic purposes in veterinary medicine. The studies described here include investigations of the residues following a single dose or multiple doses of danofloxacin. Residue depletion studies were performed to
Zedong Li et al.
The Analyst, 140(16), 5526-5535 (2015-06-17)
The integration of paper with an electrochemical device has attracted growing attention for point-of-care testing, where it is of great importance to fabricate electrodes on paper in a low-cost, easy and versatile way. In this work, we report a simple
Luisa Islas et al.
International journal of pharmaceutics, 488(1-2), 20-28 (2015-04-22)
Acrylic acid (AAc) and poly(ethylene glycol) methacrylate (PEGMA) were singly and dually grafted onto poly(vinyl chloride) (PVC) urinary catheters with the aim of preventing biofouling by endowing the catheters with the ability to load and release antimicrobial agents and to
Sunny Sun-Kin Chan et al.
Cell stem cell, 23(1), 74-85 (2018-07-07)
Derivation of functional skeletal muscle stem cells from pluripotent cells without genetic modification has proven elusive. Here we show that teratomas formed in adult skeletal muscle differentiate in vivo to produce large numbers of α7-Integrin+ VCAM-1+ myogenic progenitors. When FACS-purified and
Dianfan Li et al.
Crystal growth & design, 14(4), 2034-2047 (2014-05-08)
The lipidic mesophase or in meso method for crystallizing membrane proteins has several high profile targets to its credit and is growing in popularity. Despite its success, the method is in its infancy as far as rational crystallogenesis is concerned.
Li-Hua Mu et al.
Biological & pharmaceutical bulletin, 37(6), 1035-1041 (2014-06-03)
The aim of this study was to explore whether the ethanolic extract of Ardisia gigantifolia rhizomes (AGB-5), a traditional herbal medicine from China, could affect the proliferation of human breast adenocarcinoma (MCF-7) cells in vitro and to explore the antitumor
F Vela-Soria et al.
Talanta, 129, 209-218 (2014-08-17)
The population is continuously exposed to endocrine disrupting chemicals (EDCs). This has influenced an increase in diseases and syndromes that are more frequent nowadays. Therefore, it is necessary to develop new analytical procedures to evaluate the exposure with the ultimate
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