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Showing 1-30 of 107 results for "S9888" within Papers
Brent Veerman et al.
Bio-protocol, 7(13), e2367-e2367 (2017-07-05)
Increased amount of autoantibodies in human sera are the hallmark of autoimmune diseases ( Wang et al., 2015 ). In case of known antigen, detection of autoantibodies is done using laboratory based methods. However, in most autoimmune diseases, knowledge of
Immunofluorescent Staining of Mouse Intestinal Stem Cells.
O'Rourke KP et al.
Bio-protocol, 6, e1732-e1732 (2016)
Aleksey Gladchuk et al.
Methods and protocols, 3(2) (2020-05-08)
Fatty acids (FAs) represent an important class of metabolites, impacting on membrane building blocks and signaling compounds in cellular regulatory networks. In nature, prokaryotes are characterized with the most impressing FA structural diversity and the highest relative content of free
Daniel Fox et al.
Nature communications, 11(1), 760-760 (2020-02-08)
Inflammasomes are important for host defence against pathogens and homeostasis with commensal microbes. Here, we show non-haemolytic enterotoxin (NHE) from the neglected human foodborne pathogen Bacillus cereus is an activator of the NLRP3 inflammasome and pyroptosis. NHE is a non-redundant toxin
Yun-Xia Zhu et al.
STAR protocols, 2(3), 100728-100728 (2021-08-20)
Pancreatic islet β cells secrete insulin in a biphasic manner when sensing high blood glucose level. This protocol describes the evaluation of different phases of insulin secretion, as well as basal, glucose-stimulated and total insulin secretion abilities, thereby enabling precise
Yanlin Liu et al.
STAR protocols, 2(4), 100922-100922 (2021-11-12)
Target of rapamycin (TOR) is a central regulator in nutrient signaling. However, the innate capacity of autotrophic plants to produce carbon-related nutrients and nitrogen-related nutrients makes studying the TOR pathway difficult. Here, we describe a protocol for a liquid culture
Francesca Vallese et al.
Nature communications, 11(1), 6069-6069 (2020-11-29)
Membrane contact sites between virtually any known organelle have been documented and, in the last decades, their study received momentum due to their importance for fundamental activities of the cell and for the subtle comprehension of many human diseases. The
Abdelmoneim Eshra et al.
eLife, 10 (2021-10-07)
The Ca2+-dependence of the priming, fusion, and replenishment of synaptic vesicles are fundamental parameters controlling neurotransmitter release and synaptic plasticity. Despite intense efforts, these important steps in the synaptic vesicles' cycle remain poorly understood due to the technical challenge in
Peter Androvic et al.
Cell reports, 31(11), 107777-107777 (2020-06-20)
Ischemic stroke is a well-recognized disease of aging, yet it is unclear how the age-dependent vulnerability occurs and what are the underlying mechanisms. To address these issues, we perform a comprehensive RNA-seq analysis of aging, ischemic stroke, and their interaction
Kevin A Guttenplan et al.
Cell reports, 31(12), 107776-107776 (2020-06-25)
Glaucoma is a neurodegenerative disease that features the death of retinal ganglion cells (RGCs) in the retina, often as a result of prolonged increases in intraocular pressure. We show that preventing the formation of neuroinflammatory reactive astrocytes prevents the death
Angela Altieri et al.
Bio-protocol, 10(15), e3701-e3701 (2021-03-05)
DNA double strand breaks (DSBs) are among the most toxic lesions affecting genome integrity. DSBs are mainly repaired through non-homologous end joining (NHEJ) and homologous recombination (HR). A crucial step of the HR process is the generation, through DNA end-resection
Esteban Cruz et al.
Pharmaceutics, 13(11) (2021-11-28)
Protein aggregation constitutes a recurring complication in the manufacture and clinical use of therapeutic monoclonal antibodies (mAb) and mAb derivatives. Antibody aggregates can reduce production yield, cause immunogenic reactions, decrease the shelf-life of the pharmaceutical product and impair the capacity
Liming Tao et al.
Cell reports methods, 1(3), None-None (2021-08-04)
Cell lineage analysis aims to uncover the developmental history of an organism back to its cell of origin. Recently, novel in vivo methods utilizing genome editing enabled important insights into the cell lineages of animals. In contrast, human cell lineage remains
Jakob Kreye et al.
Cell, 183(4), 1058-1069 (2020-10-16)
The emergence of SARS-CoV-2 led to pandemic spread of coronavirus disease 2019 (COVID-19), manifesting with respiratory symptoms and multi-organ dysfunction. Detailed characterization of virus-neutralizing antibodies and target epitopes is needed to understand COVID-19 pathophysiology and guide immunization strategies. Among 598
Nicola Forte et al.
Nature communications, 12(1), 6137-6137 (2021-10-23)
The mammalian brain stores and distinguishes among episodic memories, i.e. memories formed during the personal experience, through a mechanism of pattern separation computed in the hippocampal dentate gyrus. Decision-making for food-related behaviors, such as the choice and intake of food
Randall E Burton et al.
The Journal of biological chemistry, 295(34), 12317-12327 (2020-07-16)
Bovine colostral antibodies, purified from cow's milk produced immediately after calving, have enhanced resistance to degradation by intestinal proteases relative to antibodies from human or bovine serum, making them of particular interest as orally administered therapeutic agents. However, the basis
Fariba Ghaderinezhad et al.
Scientific reports, 10(1), 13620-13620 (2020-08-14)
Analyzing electrolytes in urine, such as sodium, potassium, calcium, chloride, and nitrite, has significant diagnostic value in detecting various conditions, such as kidney disorder, urinary stone disease, urinary tract infection, and cystic fibrosis. Ideally, by regularly monitoring these ions with
Tom Flossmann et al.
Cell reports, 26(12), 3173-3182 (2019-03-21)
Synchronized activity is a universal characteristic of immature neural circuits that is essential for their developmental refinement and strongly depends on GABAergic neurotransmission. A major subpopulation of GABA-releasing interneurons (INs) expresses somatostatin (SOM) and proved critical for rhythm generation in
Christopher S Hale et al.
The journal of physical chemistry. B, 122(33), 8037-8046 (2018-08-04)
The free-solvent-based (FSB) model and osmotic pressure were used to probe the ion binding and protein hydration for self-crowded bovine serum albumin in 0.15 M NaF, NaCl, NaI, and NaSCN solutions. All experiments were conducted with solutions at pH 7.4.
Julius E Kieswich et al.
Bio-protocol, 10(18), e3751-e3751 (2021-03-05)
Cardiorenal syndrome defines a synergistic pathology of the heart and kidneys where failure of one organ causes failure in the other. The incidence of cardiovascular mortality caused by this syndrome, is 20 fold higher in the end stage renal disease
Nicholas C Wu et al.
Cell host & microbe, 25(6), 836-844 (2019-06-04)
Egg-based seasonal influenza vaccines are the major preventive countermeasure against influenza virus. However, their effectiveness can be compromised when antigenic changes arise from egg-adaptive mutations on influenza hemagglutinin (HA). The L194P mutation is commonly observed in egg-based H3N2 vaccine seed
Heterologous expression and purification of catalytic domain of CESA1 from Arabidopsis thaliana.
Vandavasi VG and O?Neill H
Bio-protocol null
Arthur H Cheng et al.
Cell reports, 26(12), 3191-3202 (2019-03-21)
Clock neurons within the mammalian suprachiasmatic nuclei (SCN) encode circadian time using interlocked transcription-translation feedback loops (TTFLs) that drive rhythmic gene expression. However, the contributions of other transcription factors outside of the circadian TTFLs to the functionality of the SCN
Hejun Liu et al.
Cell host & microbe, 29(5), 806-818 (2021-04-25)
Coronaviruses have caused several human epidemics and pandemics including the ongoing coronavirus disease 2019 (COVID-19). Prophylactic vaccines and therapeutic antibodies have already shown striking effectiveness against COVID-19. Nevertheless, concerns remain about antigenic drift in SARS-CoV-2 as well as threats from
MPM-2 Mediated Immunoprecipitation of Proteins Undergoing Proline-directed Phosphorylation.
Antonelli R and Zacchi P
Bio-protocol null
Po-Sheng Hu et al.
Nanomaterials (Basel, Switzerland), 10(3) (2020-03-04)
This research study attempts to prove the concept of the applicability of hyperthermia to treating the lysozyme amyloid fibrils (LAF)'s self-assembled fibrillary aggregates by a feedback-modulated temperature controller ranging from 26 °C to 80 °C, and separately, by near-infrared (NIR)
Lei Li et al.
Cell reports, 28(11), 2979-2995 (2019-09-12)
Munc13 proteins play several roles in regulating short-term synaptic plasticity. However, the underlying molecular mechanisms remain largely unclear. Here we report that C. elegans UNC-13L, a Munc13-1 ortholog, has three domains that inhibit synaptic vesicle (SV) exocytosis. These include the X
Siavash Fazel Darbandi et al.
Cell reports, 31(2), 107495-107495 (2020-04-16)
Tbr1 is a high-confidence autism spectrum disorder (ASD) gene encoding a transcription factor with distinct pre- and postnatal functions. Postnatally, Tbr1 conditional knockout (CKO) mutants and constitutive heterozygotes have immature dendritic spines and reduced synaptic density. Tbr1 regulates expression of
Carlos Sanchez-Martin et al.
Antioxidants & redox signaling, 34(7), 505-516 (2020-05-23)
Aims: TNF receptor-associated protein 1 (TRAP1), the mitochondrial paralog of the heat shock protein 90 (Hsp90) family of molecular chaperones, is required for neoplastic growth in several tumor cell models, where it inhibits succinate dehydrogenase (SDH) activity, thus favoring bioenergetic
Pu-Ting Dong et al.
Science advances, 7(2) (2021-02-02)
Ergosterol-targeting amphotericin B (AmB) is the first line of defense for life-threatening fungal infections. Two models have been proposed to illustrate AmB assembly in the cell membrane; one is the classical ion channel model in which AmB vertically forms transmembrane
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