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Showing 1-30 of 107 results for "S9888" within Papers
Immunofluorescent Staining of Mouse Intestinal Stem Cells.
O'Rourke KP et al.
Bio-protocol, 6, e1732-e1732 (2016)
Brent Veerman et al.
Bio-protocol, 7(13), e2367-e2367 (2017-07-05)
Increased amount of autoantibodies in human sera are the hallmark of autoimmune diseases ( Wang et al., 2015 ). In case of known antigen, detection of autoantibodies is done using laboratory based methods. However, in most autoimmune diseases, knowledge of
Aleksey Gladchuk et al.
Methods and protocols, 3(2) (2020-05-08)
Fatty acids (FAs) represent an important class of metabolites, impacting on membrane building blocks and signaling compounds in cellular regulatory networks. In nature, prokaryotes are characterized with the most impressing FA structural diversity and the highest relative content of free
Kevin A Guttenplan et al.
Cell reports, 31(12), 107776-107776 (2020-06-25)
Glaucoma is a neurodegenerative disease that features the death of retinal ganglion cells (RGCs) in the retina, often as a result of prolonged increases in intraocular pressure. We show that preventing the formation of neuroinflammatory reactive astrocytes prevents the death
Hejun Liu et al.
Immunity, 53(6), 1272-1280 (2020-11-27)
Most antibodies isolated from individuals with coronavirus disease 2019 (COVID-19) are specific to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, COVA1-16 is a relatively rare antibody that also cross-neutralizes SARS-CoV. Here, we determined a crystal structure of the COVA1-16
Francesca Vallese et al.
Nature communications, 11(1), 6069-6069 (2020-11-29)
Membrane contact sites between virtually any known organelle have been documented and, in the last decades, their study received momentum due to their importance for fundamental activities of the cell and for the subtle comprehension of many human diseases. The
Yanlin Liu et al.
STAR protocols, 2(4), 100922-100922 (2021-11-12)
Target of rapamycin (TOR) is a central regulator in nutrient signaling. However, the innate capacity of autotrophic plants to produce carbon-related nutrients and nitrogen-related nutrients makes studying the TOR pathway difficult. Here, we describe a protocol for a liquid culture
Angela Altieri et al.
Bio-protocol, 10(15), e3701-e3701 (2021-03-05)
DNA double strand breaks (DSBs) are among the most toxic lesions affecting genome integrity. DSBs are mainly repaired through non-homologous end joining (NHEJ) and homologous recombination (HR). A crucial step of the HR process is the generation, through DNA end-resection
Nicola Forte et al.
Nature communications, 12(1), 6137-6137 (2021-10-23)
The mammalian brain stores and distinguishes among episodic memories, i.e. memories formed during the personal experience, through a mechanism of pattern separation computed in the hippocampal dentate gyrus. Decision-making for food-related behaviors, such as the choice and intake of food
Jakob Kreye et al.
Cell, 183(4), 1058-1069 (2020-10-16)
The emergence of SARS-CoV-2 led to pandemic spread of coronavirus disease 2019 (COVID-19), manifesting with respiratory symptoms and multi-organ dysfunction. Detailed characterization of virus-neutralizing antibodies and target epitopes is needed to understand COVID-19 pathophysiology and guide immunization strategies. Among 598
Peter Androvic et al.
Cell reports, 31(11), 107777-107777 (2020-06-20)
Ischemic stroke is a well-recognized disease of aging, yet it is unclear how the age-dependent vulnerability occurs and what are the underlying mechanisms. To address these issues, we perform a comprehensive RNA-seq analysis of aging, ischemic stroke, and their interaction
Abdelmoneim Eshra et al.
eLife, 10 (2021-10-07)
The Ca2+-dependence of the priming, fusion, and replenishment of synaptic vesicles are fundamental parameters controlling neurotransmitter release and synaptic plasticity. Despite intense efforts, these important steps in the synaptic vesicles' cycle remain poorly understood due to the technical challenge in
Daniel Fox et al.
Nature communications, 11(1), 760-760 (2020-02-08)
Inflammasomes are important for host defence against pathogens and homeostasis with commensal microbes. Here, we show non-haemolytic enterotoxin (NHE) from the neglected human foodborne pathogen Bacillus cereus is an activator of the NLRP3 inflammasome and pyroptosis. NHE is a non-redundant toxin
Esteban Cruz et al.
Pharmaceutics, 13(11) (2021-11-28)
Protein aggregation constitutes a recurring complication in the manufacture and clinical use of therapeutic monoclonal antibodies (mAb) and mAb derivatives. Antibody aggregates can reduce production yield, cause immunogenic reactions, decrease the shelf-life of the pharmaceutical product and impair the capacity
Yun-Xia Zhu et al.
STAR protocols, 2(3), 100728-100728 (2021-08-20)
Pancreatic islet β cells secrete insulin in a biphasic manner when sensing high blood glucose level. This protocol describes the evaluation of different phases of insulin secretion, as well as basal, glucose-stimulated and total insulin secretion abilities, thereby enabling precise
Randall E Burton et al.
The Journal of biological chemistry, 295(34), 12317-12327 (2020-07-16)
Bovine colostral antibodies, purified from cow's milk produced immediately after calving, have enhanced resistance to degradation by intestinal proteases relative to antibodies from human or bovine serum, making them of particular interest as orally administered therapeutic agents. However, the basis
Fariba Ghaderinezhad et al.
Scientific reports, 10(1), 13620-13620 (2020-08-14)
Analyzing electrolytes in urine, such as sodium, potassium, calcium, chloride, and nitrite, has significant diagnostic value in detecting various conditions, such as kidney disorder, urinary stone disease, urinary tract infection, and cystic fibrosis. Ideally, by regularly monitoring these ions with
Lei Li et al.
Cell reports, 28(11), 2979-2995 (2019-09-12)
Munc13 proteins play several roles in regulating short-term synaptic plasticity. However, the underlying molecular mechanisms remain largely unclear. Here we report that C. elegans UNC-13L, a Munc13-1 ortholog, has three domains that inhibit synaptic vesicle (SV) exocytosis. These include the X
Siavash Fazel Darbandi et al.
Cell reports, 31(2), 107495-107495 (2020-04-16)
Tbr1 is a high-confidence autism spectrum disorder (ASD) gene encoding a transcription factor with distinct pre- and postnatal functions. Postnatally, Tbr1 conditional knockout (CKO) mutants and constitutive heterozygotes have immature dendritic spines and reduced synaptic density. Tbr1 regulates expression of
Christopher S Hale et al.
The journal of physical chemistry. B, 122(33), 8037-8046 (2018-08-04)
The free-solvent-based (FSB) model and osmotic pressure were used to probe the ion binding and protein hydration for self-crowded bovine serum albumin in 0.15 M NaF, NaCl, NaI, and NaSCN solutions. All experiments were conducted with solutions at pH 7.4.
Michael G Mason et al.
Nature protocols, 15(11), 3663-3677 (2020-10-03)
The complexity of current nucleic acid isolation methods limits their use outside of the modern laboratory environment. Here, we describe a fast and affordable method to purify nucleic acids from animal, plant, viral and microbial samples using a cellulose-based dipstick.
M Bokor et al.
Chemphyschem : a European journal of chemical physics and physical chemistry, 21(13), 1420-1428 (2020-05-30)
Wide-line 1 H NMR measurements were extended and all results were interpreted in a thermodynamics-based new approach on aqueous solutions of thymosin-β4 (Tβ4 ), stabilin cytoplasmic domain (CTD), and their 1 : 1 complex. Energy distributions of potential barriers controlling the motion
Joshua P Scallan et al.
Frontiers in physiology, 12, 745096-745096 (2021-10-15)
Background: Recent studies demonstrated lymphangiogenesis and expansion of draining lymph nodes during chronic inflammatory arthritis, and lymphatic dysfunction associated with collapse of draining lymph nodes in rheumatoid arthritis (RA) patients and TNF-transgenic (TNF-Tg) mice experiencing arthritic flare. As the intrinsic
Mengdie Wang et al.
Molecular biology of the cell, 30(7), 811-819 (2019-01-31)
Centrosome abnormalities are emerging hallmarks of cancer. The overproduction of centrosomes (known as centrosome amplification) has been reported in a variety of cancers and is currently being explored as a promising target for therapy. However, to understand different types of
Joëlle J E Janssen et al.
Scientific reports, 11(1), 1662-1662 (2021-01-20)
Analyzing metabolism of peripheral blood mononuclear cells (PBMCs) provides key opportunities to study the pathophysiology of several diseases, such as type 2 diabetes, obesity and cancer. Extracellular flux (XF) assays provide dynamic metabolic analysis of living cells that can capture
Patryk Ludzia et al.
Structure (London, England : 1993), 29(9), 1014-1028 (2021-04-30)
The kinetochore is the macromolecular machinery that drives chromosome segregation by interacting with spindle microtubules. Kinetoplastids (such as Trypanosoma brucei), a group of evolutionarily divergent eukaryotes, have a unique set of kinetochore proteins that lack any significant homology to canonical
Izabela N Faria Gomes et al.
Molecules (Basel, Switzerland), 24(21) (2019-11-07)
Plant-based compounds are an option to explore and perhaps overcome the limitations of current antitumor treatments. Annona coriacea Mart. is a plant with a broad spectrum of biological activities, but its antitumor activity is still unclear. The purpose of our
Gregory J Baker et al.
Cell systems, 11(3), 272-285 (2020-09-09)
Accurately profiling systemic immune responses to cancer initiation and progression is necessary for understanding tumor surveillance and, ultimately, improving therapy. Here, we describe the SYLARAS software tool (systemic lymphoid architecture response assessment) and a dataset collected with SYLARAS that describes
Anna Piaszyk-Borychowska et al.
Frontiers in immunology, 10, 1253-1253 (2019-06-25)
Atherosclerosis is a chronic inflammatory disease of the blood vessels, characterized by atherosclerotic lesion formation. Vascular Smooth Muscle Cells (VSMC), macrophages (MΦ), and dendritic cells (DC) play a crucial role in vascular inflammation and atherosclerosis. Interferon (IFN)α, IFNγ, and Toll-like
Meng Yuan et al.
Cell host & microbe, 25(6), 873-883 (2019-06-14)
The fusion peptide (FP) of HIV-1 envelope glycoprotein (Env) is essential for mediating viral entry. Detection of broadly neutralizing antibodies (bnAbs) that interact with the FP has revealed it as a site of vulnerability. We delineate X-ray and cryo-electron microscopy
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