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Showing 1-30 of 183 results for "T6567" within Papers
Lucia F Zacchi et al.
Journal of proteome research, 19(5), 2149-2158 (2020-03-26)
Proteomic analysis of bioreactor supernatants can inform on cellular metabolic status, viability, and productivity, as well as product quality, which can in turn help optimize bioreactor operation. Incubating mammalian cells in bioreactors requires the addition of polymeric surfactants such as
Masayuki Shimoda et al.
Cell transplantation, 21(2-3), 465-471 (2012-07-17)
Porcine islets are considered to be a promising resource for xenotransplantation. However, it is difficult to isolate porcine islets because of the marked fragility and rapid dissociation. Endogenous trypsin is one of the main factors to damage islets during the
Matthew A Lauber et al.
Analytical chemistry, 82(18), 7736-7743 (2010-08-28)
A novel bifunctional thioimidate cross-linking reagent (diethyl suberthioimidate) that modifies amines without sacrificing their native basicity is developed. Intermolecular cross-linking of neurotensin and intramolecular cross-linking of cytochrome c under physiological conditions is investigated with this reagent. Because it does not
Lorenzo Bonaguro et al.
Nature immunology, 21(12), 1517-1527 (2020-11-11)
CRELD1 is a pivotal factor for heart development, the function of which is unknown in adult life. We here provide evidence that CRELD1 is an important gatekeeper of immune system homeostasis. Exploiting expression variance in large human cohorts contrasting individuals
Lan Zhang et al.
Proteomics, 16(1), 12-28 (2015-10-06)
Menopause is one of the crucial physiological events during the life of a woman. Transition of menopause status is accompanied by increased risks of various health problems such as osteoporosis. Peripheral blood monocytes can differentiate into osteoclasts and produce cytokines
Peter Karuso et al.
Journal of proteome research, 7(1), 361-366 (2007-12-07)
Ensuring that proteolytic digestions are complete before submitting samples for downstream proteomic analyses is important, as failure or partial digestion can waste valuable instrument time and make results difficult to interpret. Conversely, overdigestion can also be problematic, such as when
Eileen Ke et al.
Pancreas, 38(2), e33-e42 (2009-01-13)
There are currently no diagnostic indicators that are consistently reliable, obtainable, and conclusive for diagnosing and risk-stratifying pancreatic cysts. Proteomic analyses were performed to explore pancreatic cyst fluids to yield effective diagnostic biomarkers. We have prospectively recruited 20 research participants
Tamiza Nanji et al.
Autophagy, 13(12), 2018-2027 (2017-10-05)
Although the human ULK complex mediates phagophore initiation similar to the budding yeast Saccharomyces cerevisiae Atg1 complex, this complex contains ATG101 but not Atg29 and Atg31. Here, we analyzed the fission yeast Schizosaccharomyces pombe Atg1 complex, which has a subunit
Quantitative proteomics and phosphoproteomics of urinary extracellular vesicles define putative diagnostic biosignatures for Parkinson's disease.
Hadisurya, et al.
Communications medicine, 3, 64-64 (2023)
Onrapak Reamtong et al.
Acta tropica, 202, 105247-105247 (2019-11-02)
Schistosoma mekongi is a causative agent of human schistosomiasis. There is limited knowledge of the molecular biology of S. mekongi and very few studies have examined drug targets, vaccine candidates and diagnostic biomarkers for S. mekongi. To explore the biology
Zdeněk Perutka et al.
Molecules (Basel, Switzerland), 23(10) (2018-10-17)
Trypsin is the protease of choice for protein sample digestion in proteomics. The most typical active forms are the single-chain β-trypsin and the two-chain α-trypsin, which is produced by a limited autolysis of β-trypsin. An additional intra-chain split leads to
Ronivaldo Rodrigues da Silva et al.
Applied biochemistry and biotechnology, 187(4), 1158-1172 (2018-09-05)
The fungal genus Pyrenochaetopsis has received particular attention because of its different lifestyles, such as numerous plant pathogenic, saprophytic, and endophytic species. Its ability to infect plant cells relies heavily upon secreted peptidases. Here, we investigated the biochemical properties and
Ronivaldo Rodrigues da Silva et al.
Journal of biotechnology, 294, 73-80 (2019-02-24)
β-glucosidases are glycoside hydrolases that-particularly those from filamentous fungi-have been extensively explored in cellulose fiber saccharification and wine quality improvement. However, these enzymes from yeast have been poorly studied. In this study, an ethanol-glucose tolerant β-glucosidase that is secreted by
Devon Radford et al.
Frontiers in microbiology, 9, 2123-2123 (2018-09-27)
With the alarming proliferation of antibiotic resistance, it is important to understand the de novo development of bacterial adaptation to antibiotics in formerly susceptible lineages, in the absence of external genetic input from existing resistance pools. A strain of ceftiofur
Nathrada Khantavee et al.
Veterinary dermatology, 32(4), 338-e94-338-e94 (2021-05-18)
Dermatophagoides farinae (Der f) is a common allergen in dogs with atopic dermatitis (AD). However, the relevant components of Der f require further investigation. We aimed to provide data on the immunoglobulin (Ig)E-binding specific components of Der f for further
Ralph Adams et al.
Frontiers in immunology, 14, 1170357-1170357 (2023-05-30)
Serum albumin binding is an established mechanism to extend the serum half-life of antibody fragments and peptides. The cysteine rich knob domains, isolated from bovine antibody ultralong CDRH3, are the smallest single chain antibody fragments described to date and versatile
Supeecha Kumkate et al.
Toxins, 12(1) (2020-01-23)
The two venomous pit vipers, Trimeresurus macrops and T. hageni, are distributed throughout Thailand, although their abundance varies among different areas. No species-specific antivenom is available for their bite victims, and the only recorded treatment method is a horse antivenom
Kenichi Yamaguchi
Methods in molecular biology (Clifton, N.J.), 775, 241-264 (2011-08-25)
Proteomics of chloroplast ribosomes in spinach and Chlamydomonas revealed unique protein composition and structures of plastid ribosomes. These studies have suggested the presence of some ribosomal proteins unique to plastid ribosomes which may be involved in plastid-unique translation regulation. Considering
Javier Aguilera et al.
The Journal of biological chemistry, 295(17), 5785-5794 (2020-03-15)
The Mycobacterium tuberculosis virulence factor EsxA and its chaperone EsxB are secreted as a heterodimer (EsxA:B) and are crucial for mycobacterial escape from phagosomes and cytosolic translocation. Current findings support the idea that for EsxA to interact with host membranes
Xiang-Rong Li et al.
Cell death discovery, 7(1), 11-11 (2021-01-16)
Besides their original regulating roles in the brain, spinal cord, retina, and peripheral nervous system for mediating fast excitatory synaptic transmission, glutamate receptors consisting of metabotropic glutamate receptors (GluRs) and ionotropic glutamate receptors (iGluRs) have emerged to have a critical
Sandra Backes et al.
Cell reports, 35(1), 108936-108936 (2021-04-08)
Most mitochondrial proteins are synthesized as precursors in the cytosol and post-translationally transported into mitochondria. The mitochondrial surface protein Tom70 acts at the interface of the cytosol and mitochondria. In vitro import experiments identified Tom70 as targeting receptor, particularly for hydrophobic
Gints Kalnins et al.
Nature communications, 11(1), 388-388 (2020-01-22)
Bacterial microcompartments (BMCs) are prokaryotic organelles consisting of a protein shell and an encapsulated enzymatic core. BMCs are involved in several biochemical processes, such as choline, glycerol and ethanolamine degradation and carbon fixation. Since non-native enzymes can also be encapsulated
Zdeněk Perutka et al.
Molecules (Basel, Switzerland), 23(10) (2018-10-17)
Trypsin is the protease of choice for protein sample digestion in proteomics. The most typical active forms are the single-chain β-trypsin and the two-chain α-trypsin, which is produced by a limited autolysis of β-trypsin. An additional intra-chain split leads to
Lorena Caixeta de Oliveira Simões et al.
Applied biochemistry and biotechnology, 188(4), 991-1008 (2019-02-15)
Xylanases are enzymes that act in the depolymerization of xylan and that can be used in the food industry, the paper industry, and for bioenergy, among other uses. In this context, particular emphasis is devoted to xylooligosaccharides (XOS) that act
Fei-Yan Deng et al.
Molecular & cellular proteomics : MCP, 10(11), M111-M111 (2011-08-06)
Low bone mineral density (BMD) is a risk factor of osteoporosis and has strong genetic determination. Genes influencing BMD and fundamental mechanisms leading to osteoporosis have yet to be fully determined. Peripheral blood monocytes (PBM) are potential osteoclast precursors, which
Douglas E Vetter et al.
Methods in molecular biology (Clifton, N.J.), 493, 345-366 (2008-10-08)
Modern biologists have at their disposal a large array of techniques used to assess the existence and relative or absolute quantity of any molecule of interest in a sample. However, implementing most of these procedures can be a daunting task
Bin-Tong Yang et al.
Annals of the New York Academy of Sciences, 1486(1), 58-75 (2020-10-04)
Aeromonas veronii is an important zoonotic and aquatic pathogen. An increasing number of reports indicate that it has caused substantial economic losses in the aquaculture industry, in addition to threatening human health. However, little is known about its pathogenesis. Exploration
Jeonghoon Lee et al.
Journal of the American Society for Mass Spectrometry, 19(7), 964-972 (2008-05-16)
An automated proteolytic digestion bioreactor and droplet deposition system was constructed with a plastic microfluidic device for off-line interfacing to matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The microfluidic chips were fabricated in poly(methyl methacrylate) (PMMA), using a
Dylan J Harney et al.
Molecular & cellular proteomics : MCP, 18(9), 1899-1915 (2019-07-17)
Unbiased and sensitive quantification of low abundance small proteins in human plasma (e.g. hormones, immune factors, metabolic regulators) remains an unmet need. These small protein factors are typically analyzed individually and using antibodies that can lack specificity. Mass spectrometry (MS)-based
Yan S Xu et al.
Scientific reports, 6, 39517-39517 (2016-12-23)
Cytoplasmic STAT3, after activation by growth factors, translocates to different subcellular compartments, including nuclei and mitochondria, where it carries out different biological functions. However, the precise mechanism by which STAT3 undergoes mitochondrial translocation and subsequently regulates the tricarboxylic acid (TCA)
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