prepared 1X TE buffer at 2-8ºC. Warm to room temperature before use. Discard
1X TE if it becomes cloudy or contaminated.
Storage / Handling: When stored at 2-8°C, the 10X TE buffer is stable up
Store at –20 °C between uses.
2. 1X TE Buffer
Add 1 volume of 100X TE to 99 volumes of
molecular biology grade water as needed (e.g.
10 µl of 100X TE buffer to 990 µl water).
Store at –20 °C between uses.
2. 1X TE Buffer
Add 1 volume of 100X TE to 99 volumes of
molecular biology grade water as needed (e.g.
10 µl of 100X TE buffer to 990 µl water).
Preparation
1. Prepare 1X TE buffer. If using 100X TE buffer, dilute
the concentrate 1:100 with molecular biology grade
water to prepare 1X TE buffer.
2. Add 56.25 µl of 1X TE buffer to the
Preparation
1. Prepare 1X TE buffer. If using 100X TE buffer, dilute
the concentrate 1:100 with molecular biology grade
water to prepare 1X TE buffer.
2. Add 56.25 µl of 1X TE buffer to the
supplied at an initial
concentration of 5 µM in 0.1× TE buffer (1 mM Tris,
pH 8.0, 0.1 mM EDTA).
Reagents Required but Not Provided
• 1× TE buffer (10 mM Tris, pH 8.0, 1.0 mM EDTA)
• 5 M
supplied at an
initial concentration of 5 µM in 1× TE buffer (10 mM
Tris, pH 8.0, 1.0 mM EDTA).
Reagents Required but Not Provided
• 1× TE buffer
• 5 M NaOH
• 3 M potassium/5 M acetate: To 60
supplied at an
initial concentration of 5 µM in 1X TE buffer (10 mM
Tris, pH 8.0, 1.0 mM EDTA).
Reagents Required but Not Provided
• 1X TE buffer
• 5 M NaOH
• 3 M potassium/5 M acetate: To
supplied at an initial
concentration of 5 µM in 0.1× TE buffer (1 mM Tris,
pH 8.0, 0.1 mM EDTA).
Reagents Required but Not Provided
• 1× TE buffer (10 mM Tris, pH 8.0, 1.0 mM EDTA)
• 5 M
Reagents Required But Not
Provided
• Agarose, Product Code A 9539
• 1X TE (Tris-EDTA) buffer, prepared from 100X TE
buffer, Product Code T 9285, or molecular biology
reagent water, Product Code W
using a Tecan Genesis® Te-MOTM Multi-
Channel Pipetting Option, a stand-alone 96 channel pipettor.
• 20 µL aliquots of the PCR samples were diluted with
80 µL TE buffer
• 100 µL from each well
supplied at an initial
concentration of 5 µM in 1X TE buffer (10 mM Tris,
1.0 mM EDTA, pH 8.0).
Reagents Required but Not Provided
• 1X TE buffer
• 5 M NaOH
• 3 M potassium/5 M acetate: To