supplied at an
initial concentration of 5 µM in 1X TE buffer (10 mM
Tris, pH 8.0, 1.0 mM EDTA).
Reagents Required but Not Provided
• 1X TE buffer
• 5 M NaOH
• 3 M potassium/5 M acetate: To
supplied at an
initial concentration of 5 µM in 1× TE buffer (10 mM
Tris, pH 8.0, 1.0 mM EDTA).
Reagents Required but Not Provided
• 1× TE buffer
• 5 M NaOH
• 3 M potassium/5 M acetate: To 60
supplied at an initial
concentration of 5 µM in 0.1× TE buffer (1 mM Tris,
pH 8.0, 0.1 mM EDTA).
Reagents Required but Not Provided
• 1× TE buffer (10 mM Tris, pH 8.0, 1.0 mM EDTA)
• 5 M
K digestion. Over staining is
observed with EDTA pH 8.0 and TE Buffer pH 9.0, no signal is
detected without Epitope retrieval.
IHC-Paraffin Protocol:
IHC-Select, HRP-DAB (Brief Outline):
supplied at an initial
concentration of 5 µM in 0.1× TE buffer (1 mM Tris,
pH 8.0, 0.1 mM EDTA).
Reagents Required but Not Provided
• 1× TE buffer (10 mM Tris, pH 8.0, 1.0 mM EDTA)
• 5 M
Reagents Required But Not
Provided
• Agarose, Product Code A 9539
• 1X TE (Tris-EDTA) buffer, prepared from 100X TE
buffer, Product Code T 9285, or molecular biology
reagent water, Product Code W
using a Tecan Genesis® Te-MOTM Multi-
Channel Pipetting Option, a stand-alone 96 channel pipettor.
• 20 µL aliquots of the PCR samples were diluted with
80 µL TE buffer
• 100 µL from each well
supplied at an initial
concentration of 5 µM in 1X TE buffer (10 mM Tris,
1.0 mM EDTA, pH 8.0).
Reagents Required but Not Provided
• 1X TE buffer
• 5 M NaOH
• 3 M potassium/5 M acetate: To
of DNA-standard in 100 µl TE buffer pH 7.5
3. Dilute the unknown sample DNA in 100 µl TE buffer pH 7.5
4. Working solution: Dilute 4 µl Nancy-520 in 10 ml TE buffer pH
Over staining or no staining
occurred with TE Buffer, pH 9.0 and no HIER, respectively.
IHC-Paraffin Protocol:
IHC-Select, HRP-DAB (Brief Outline):
Blocking Reagent for 5 min.
Primary Antibody