Specification Sheet
Product Name
MystiCq® microRNA cDNA Synthesis Mix
Product Number
MIRRT
Product Brand
SIGMA
CAS Number
Molecular Weight
Test | Specification |
|---|---|
| qScript microRNA cDNA Synthesis Kit | Must have detection of microRNA from 100 ng to 10 pg.Correlation of determination (R2) ≥ 0.990 from Ct standard curve analysis.Slope from Ct standard curve analysis between -3.20 and -3.75.The +PAP reactions must show a ≥ 11 Ct difference from the “no-PAP” reactions.There must be at least three negative template controls with “undetermined” values. |
| DNase | DNase activity must be below the detectable limits of 100 pg DNase I equivalent as assayed using a fluorogenic substrate following a 1 hour incubation at 37°C with each kit component at 1X concentration. |
| RNase | RNase activity must be below the detectable limits of 1 pg RNase A equivalent as assayed using a fluorogenic substrate following a 1 hour incubation at 37°C with each kit component at 1X concentration |
| Expiration Date | Report result |