Specification Sheet
Product Name
GenElute™-E Single Spin Cell Culture DNA Kit Reagents and consumables for various cell culture sample types. 10, 50 or 250 purifications.
Product Number
ec400
Product Brand
sigma
CAS Number
Molecular Weight
Test | Specification |
|---|---|
| Expiry | Report result |
| Yield/mg tissue (µg/mg) | > 2 µg DNA / mg tissue |
| Concentration (ng/µl) | ≥ 25 ng/µL |
| PCR functionality | ≤ 28 Ct |
| Ratio 260/280 | 1.4-2.1 |
| Eluate volume | 80-105 µL |
| Eluate OD 230 | < 0.2 |
| Appearance in Agarose Gel | Genomic DNA band clearly visible |
| Clogging of column | No clogging observed |
| Colour-free eluate | No reddish colour in eluate |
| Spin Columns (Lot 9004) | Pass |
| Void OD A230 | 0.1 |
| Eluate OD A230 | 0.07 mL |
| Resin concentration (mL) | 6.75 mL |
| Salt depletion / conductivity [% or µS/cm] | 96% /< 200 µS/cm |
| PCR (FAM) | Ct 29 |
| PCR (VIC) | Ct 29 |
| DNA recovery rate (%) | 0.763 |
| Agarose gel | Depletion visible maximum in 300bp band |
| 1x Tris Buffer (8980) | Pass |
| pH target value | pH 8.5 |