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  • Direct liquid chromatography method for retinol, alpha- and gamma-tocopherols in rat plasma.

Direct liquid chromatography method for retinol, alpha- and gamma-tocopherols in rat plasma.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (2003-12-31)
F J Rupérez, M Mach, C Barbas
PMID14698258
ABSTRACT

An HPLC method for Vitamins A and E in rat plasma has been developed. The main goals of the method are the small amount of sample, 50 microl, and the direct extraction of analytes in one step with acetone, which is a solvent compatible with the reverse-phase mobile phases. Recoveries, as compared with classical and more tedious methods, were near 100%. The method employs a Supelco Discovery C18 column and methanol/water (95:5, v/v) as mobile phase. After being developed, the method was validated following ICH guidelines, with UV, fluorescence and electrochemical detectors. It proved to be selective, lineal, accurate and precise. This method greatly simplifies sample treatment and that is a critical point when working with a large number of samples.

MATERIALS
Product Number
Brand
Product Description
569232-U
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 12.5 cm × 4.6 mm
504955
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
504947
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 5 cm × 4.6 mm
504971
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
505137
Supelco
Discovery® C18 Supelguard Guard Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm, pkg of 2 ea
505161
Supelco
Discovery® C18 Supelguard Guard Cartridge, Kit, 5 μm particle size, L × I.D. 2 cm × 2.1 mm
505129
Supelco
Discovery® C18 Supelguard Guard Cartridge, Kit, 5 μm particle size, L × I.D. 2 cm × 4 mm
50494721
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 5 cm × 2.1 mm
50495521
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 2.1 mm
505188
Supelco
Discovery® C18 Supelguard Guard Cartridge, 5 μm particle size, L × I.D. 2 cm × 2.1 mm, pkg of 2 ea
569222-U
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 10 cm × 4 mm
569226-U
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 21.2 mm
569224-U
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 10 mm
569231-U
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 12.5 cm × 4 mm
59576-U
Supelco
Discovery® C18 Supelguard Guard Cartridge, 5 μm particle size, L × I.D. 2 cm × 3 mm, pkg of 2 ea
504971-40
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4 mm
569220-U
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 10 cm × 2.1 mm
569229-U
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 12.5 cm × 2.1 mm
59575-U
Supelco
Discovery® C18 Supelguard Guard Cartridge, Kit, 5 μm particle size, L × I.D. 2 cm × 3 mm
504955-40
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4 mm
569223-U
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 10 cm × 4.6 mm
504955-30
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 3 mm
504971-30
Supelco
Discovery® C18 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 3 mm