- Establishment of human embryonic stem cell line Amicqui-2 using poor-quality embryos from Mexican population.
Establishment of human embryonic stem cell line Amicqui-2 using poor-quality embryos from Mexican population.
Stem cell research (2019-01-06)
Daniela Ávila-González, Omar Martínez-Alarcón, Guadalupe García-López, Néstor Emmanuel Díaz-Martínez, Guadalupe Razo-Aguilera, María Yolotzin Valdespino-Vázquez, Elsa Romelia Moreno-Verduzco, Eva Vega-Hernández, Juan Carlos Regalado-Hernández, Julio Francisco De la Jara-Díaz, Anayansi Molina-Hernández, Héctor Flores-Herrera, Wendy Portillo, Néstor Fabián Díaz
PMID30611019
ABSTRACT
Although investigation with human embryonic stem cells (HESC) is not decreasing, the derivation of new lines has been diminished. The preeminence of only a few HESC lines in research is accompanied by lack of universal applicability of results as well as by genetic under-representation. We previously reported the derivation of one line with male karyotype from Mexican population. Here, we derived one HESC line (Amicqui-2) with female karyotype from poor-quality embryos. These line comply the pluripotent requirements (normal karyotype, detection of pluripotency-associated markers, mycoplasma test and teratoma formation) and could be a valuable model for studying diseases specific to under-represented population.