- Staphylococcus aureus cell wall stress stimulon gene-lacZ fusion strains: potential for use in screening for cell wall-active antimicrobials.
Staphylococcus aureus cell wall stress stimulon gene-lacZ fusion strains: potential for use in screening for cell wall-active antimicrobials.
Antimicrobial agents and chemotherapy (2008-06-11)
Rebecca Steidl, Stacy Pearson, Robert E Stephenson, Nagender Ledala, Sutthirat Sitthisak, Brian J Wilkinson, Radheshyam K Jayaswal
PMID18541730
ABSTRACT
lacZ fusion strains were constructed using the promoters of five cell wall stress stimulon genes: pbp2, tcaA, vraSR, sgtB, and lytR. All fusion strains were induced only in the presence of cell wall-active antibiotics, suggesting the potential of these strains for use in high-throughput screening for new cell wall-active agents.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Rifampicin, suitable for plant cell culture, BioReagent, ≥95% (HPLC), powder or crystals