- A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM).
A protocol for metabolic characterization of human induced pluripotent stem cell-derived cardiomyocytes (iPS-CM).
MethodsX (2020-03-07)
Alisha House, Erica Fatica, Rohan Shah, Jared Stergar, Ryan Pearce, Yana Sandlers
PMID32140435
ABSTRACT
Recent advances in human induced pluripotent stem cell-derived cardiomyocytes (iPSCM) field offer a novel platform for modeling cardiac metabolism, heart diseases drug candidates screening and cardiac toxicity assessments. These workflows require a fully functional characterization of iPSCMs. Here we report a step by step protocol for iPSCM metabolic characterization. The described assays cover analysis of small metabolites involved in a vital metabolic pathways.
MATERIALS
Product Number
Brand
Product Description
Supelco
Hydroxylamine Hydrochloride, Meets ACS Specifications, Meets Reagent Specifications for testing USP/NF monographs GR ACS