Merck
CN

Chitin Extraction and Content Measurement in Magnaporthe oryzae.

Bio-protocol (2017-03-05)
Xinyu Liu, Zhengguang Zhang
ABSTRACT

Chitin is a linear polysaccharide composed of β (1→4)-linked N-acetylglucosamine (GlcNAc) residues. In fungi, chitin is an important component of the cell wall. Here, we provide a protocol to measure the chitin content of fungal cells using Magnaporthe oryzae as an example.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Chitinase from Streptomyces griseus, lyophilized powder (essentially salt free), ≥200 units/g solid