Extraction of Soluble and Insoluble Protein Fractions from Mouse Brains and Spinal Cords.

Extraction of Soluble and Insoluble Protein Fractions from Mouse Brains and Spinal Cords.

Bio-protocol (2017-08-05)

Oliver Wirths

ABSTRACT

The current protocol details the preparation of soluble and insoluble protein lysates from mouse brain or spinal cord samples. In detail, tissue homogenization and sequential protein extraction are described. This procedure yields soluble and insoluble protein extracts that can be further processed in down-stream applications like ELISA or Western blotting.

MATERIALS

Product Number

Brand

Product Description

P0044

Sigma-Aldrich

Phosphatase Inhibitor Cocktail 3, DMSO solution

E1014

Millipore

Benzonase^® Nuclease, ≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

04693159001

Roche

cOmplete^™, Mini, EDTA-free Protease Inhibitor Cocktail, Tablets provided in EASYpacks