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  • Multicenter collaborative study for standardization of Candida albicans genotyping using a polymorphic microsatellite marker.

Multicenter collaborative study for standardization of Candida albicans genotyping using a polymorphic microsatellite marker.

Journal of clinical microbiology (2010-04-30)
Dea Garcia-Hermoso, Donna M MacCallum, Timothy J Lott, Paula Sampaio, Maria-José Buitrago Serna, Fréderic Grenouillet, Corné H W Klaassen, Stéphane Bretagne
ABSTRACT

Microsatellite-based genotyping for Candida albicans can give discrepant results between laboratories when expressed in fragment sizes, because their determination depends on electrophoretic conditions. The interlaboratory reproducibility was assessed in six laboratories provided with an allelic ladder. Despite variations in size determinations, alleles were correctly assigned, making data transportable between laboratories.