- Integrated proteomic analysis of post-translational modifications by serial enrichment.
Integrated proteomic analysis of post-translational modifications by serial enrichment.
Nature methods (2013-06-12)
Philipp Mertins, Jana W Qiao, Jinal Patel, Namrata D Udeshi, Karl R Clauser, D R Mani, Michael W Burgess, Michael A Gillette, Jacob D Jaffe, Steven A Carr
PMID23749302
ABSTRACT
We report a mass spectrometry-based method for the integrated analysis of protein expression, phosphorylation, ubiquitination and acetylation by serial enrichments of different post-translational modifications (SEPTM) from the same biological sample. This technology enabled quantitative analysis of nearly 8,000 proteins and more than 20,000 phosphorylation, 15,000 ubiquitination and 3,000 acetylation sites per experiment, generating a holistic view of cellular signal transduction pathways as exemplified by analysis of bortezomib-treated human leukemia cells.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Phosphatase Inhibitor Cocktail 2, aqueous solution (dark coloration may develop upon storage, which does not affect the activity)