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  • Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).

Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition).

European journal of immunology (2019-10-22)
Andrea Cossarizza, Hyun-Dong Chang, Andreas Radbruch, Andreas Acs, Dieter Adam, Sabine Adam-Klages, William W Agace, Nima Aghaeepour, Mübeccel Akdis, Matthieu Allez, Larissa Nogueira Almeida, Giorgia Alvisi, Graham Anderson, Immanuel Andrä, Francesco Annunziato, Achille Anselmo, Petra Bacher, Cosima T Baldari, Sudipto Bari, Vincenzo Barnaba, Joana Barros-Martins, Luca Battistini, Wolfgang Bauer, Sabine Baumgart, Nicole Baumgarth, Dirk Baumjohann, Bianka Baying, Mary Bebawy, Burkhard Becher, Wolfgang Beisker, Vladimir Benes, Rudi Beyaert, Alfonso Blanco, Dominic A Boardman, Christian Bogdan, Jessica G Borger, Giovanna Borsellino, Philip E Boulais, Jolene A Bradford, Dirk Brenner, Ryan R Brinkman, Anna E S Brooks, Dirk H Busch, Martin Büscher, Timothy P Bushnell, Federica Calzetti, Garth Cameron, Ilenia Cammarata, Xuetao Cao, Susanna L Cardell, Stefano Casola, Marco A Cassatella, Andrea Cavani, Antonio Celada, Lucienne Chatenoud, Pratip K Chattopadhyay, Sue Chow, Eleni Christakou, Luka Čičin-Šain, Mario Clerici, Federico S Colombo, Laura Cook, Anne Cooke, Andrea M Cooper, Alexandra J Corbett, Antonio Cosma, Lorenzo Cosmi, Pierre G Coulie, Ana Cumano, Ljiljana Cvetkovic, Van Duc Dang, Chantip Dang-Heine, Martin S Davey, Derek Davies, Sara De Biasi, Genny Del Zotto, Gelo Victoriano Dela Cruz, Michael Delacher, Silvia Della Bella, Paolo Dellabona, Günnur Deniz, Mark Dessing, James P Di Santo, Andreas Diefenbach, Francesco Dieli, Andreas Dolf, Thomas Dörner, Regine J Dress, Diana Dudziak, Michael Dustin, Charles-Antoine Dutertre, Friederike Ebner, Sidonia B G Eckle, Matthias Edinger, Pascale Eede, Götz R A Ehrhardt, Marcus Eich, Pablo Engel, Britta Engelhardt, Anna Erdei
ABSTRACT

These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all written and peer-reviewed by leading experts in the field, making this an essential research companion.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Propidium iodide, ≥94.0% (HPLC)
Sigma-Aldrich
DL-Dithiothreitol solution, BioUltra, Molecular Biology, ~1 M in H2O
Sigma-Aldrich
Dasatinib
Sigma-Aldrich
HEPES solution, 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture