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  • Native and fluorescent dye-dependent single-DNA molecule microchip dynamics as measured by differential interference contrast microscopy.

Native and fluorescent dye-dependent single-DNA molecule microchip dynamics as measured by differential interference contrast microscopy.

Chemical communications (Cambridge, England) (2011-07-14)
Doori Oh, Seungah Lee, Seong Ho Kang
ABSTRACT

In a free solution of 10.0 mM Gly-Gly (pH 8.2), the flow directions of native-DNA and DNA molecules intercalated with the fluorescent dye YOYO-1 were reversed in the microchip channel. These results clearly showed that in a confined space and under specific environmental conditions, the fluorescent dye modified the original properties and behavior of the native-DNA molecule.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Gly-Gly, BioXtra, ≥99.0% (NT)
Sigma-Aldrich
Gly-Gly, ≥99% (titration)
Sigma-Aldrich
Gly-Gly, BioUltra, ≥99.5% (NT)
Sigma-Aldrich
Gly-Gly, BioPerformance Certified, suitable for cell culture, ≥99%
Sigma-Aldrich
Gly-Gly, Vetec, reagent grade, 99%