- Assessing Neuronal Excitability on a Fluorometric Imaging Plate Reader (FLIPR) Following a Defined Electrostimulation Paradigm.
Assessing Neuronal Excitability on a Fluorometric Imaging Plate Reader (FLIPR) Following a Defined Electrostimulation Paradigm.
Methods in molecular biology (Clifton, N.J.) (2019-05-28)
Valeria Kizner, Sandra Fischer, Benjamin Jähnke, Maximilian Naujock
PMID31124119
ABSTRACT
FLIPR-based calcium assay enables the detection and characterization of neuronal excitability by using electrical field stimulation to evoke and record action potential-driven calcium transients in induced pluripotent stem cell (iPSC)-derived cortical forebrain neurons. Here we describe high throughput measurement of neuronal excitability with a defined electrostimulation paradigm in a 384-well plate format using FLIPR.
MATERIALS
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Sigma-Aldrich
Laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane, 1-2 mg/mL in Tris-buffered saline, 0.2 μm filtered, BioReagent, suitable for cell culture