- Molecular and cellular approaches for establishment of an in vitro spermatogenesis system.
Molecular and cellular approaches for establishment of an in vitro spermatogenesis system.
Methods in molecular biology (Clifton, N.J.) (2004-03-24)
Karim Nayernia, Wolfgang Engel
PMID15037792
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Collagenase from Clostridium histolyticum, 0.2 μm filtered, suitable for release of physiologically active rat hepatocytes, Type IV-S, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid
Sigma-Aldrich
Trypsin-EDTA solution, 0.25%, sterile-filtered, BioReagent, suitable for cell culture, 2.5 g porcine trypsin and 0.2 g EDTA, 4Na per liter of Hanks′ Balanced Salt Solution with phenol red