- Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of human parotid salivary proteins: comparison of dansylation, coomassie blue R-250 and silver detection methods.
Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of human parotid salivary proteins: comparison of dansylation, coomassie blue R-250 and silver detection methods.
Electrophoresis (1996-03-01)
J A Beeley, F Newman, P H Wilson, I C Shimmin
PMID8740168
ABSTRACT
Detection of human parotid salivary proteins by dansylation and UV-transillumination after sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) has been compared with Coomassie Blue R-250 and silver staining procedures. Dansylation gives superior results in terms of both resolution and sensitivity, especially with basic proline-rich proteins (PRPs).
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Brilliant Blue R Staining Solution, suitable for (for immunoelectrophoresis protein staining)