- DNA digestion to deoxyribonucleoside: a simplified one-step procedure.
DNA digestion to deoxyribonucleoside: a simplified one-step procedure.
Analytical biochemistry (2007-11-22)
Eoin P Quinlivan, Jesse F Gregory
PMID18028864
ABSTRACT
We present a simple and inexpensive 'one-step' protocol for the hydrolysis of DNA to deoxyribonucleosides. Unlike the older DNA hydrolysis protocol which is cumbersome and labor intensive, this new protocol is ideal for high-throughput assays and is suitable automation. Using this protocol we were able to hydrolyze several hundred samples within an 8-hour period. The new protocol is fully compatible with LC-MS/MS and gives similar recoveries for all five major deoxyribonucleosides when compared to the older protocol.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Benzonase® Nuclease, ultrapure, ≥250 units/μL, ≥99% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution, ultrapure grade
Millipore
Benzonase® Nuclease, ≥250 units/μL, ≥90% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution