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  • Membraneless glucose/O2 microfluidic biofuel cells using covalently bound enzymes.

Membraneless glucose/O2 microfluidic biofuel cells using covalently bound enzymes.

Chemical communications (Cambridge, England) (2013-01-04)
T Beneyton, I Putu Mahendra Wijaya, C Ben Salem, A D Griffiths, V Taly
ABSTRACT

We developed a new covalent enzyme immobilization technique compatible with lithography processes. Carbon nanotube electrodes patterned on glass slides were used to create Y-shaped membraneless glucose/O(2) microfluidic biofuel cells. An original extremophilic laccase, CotA from Bacillus subtilis, was used in the cathodic compartment of these miniaturized biofuel cells.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Glucose Oxidase from Aspergillus niger, Type VII, lyophilized powder, ≥100,000 units/g solid (without added oxygen)
Sigma-Aldrich
Glucose Oxidase from Aspergillus niger, Type X-S, lyophilized powder, 100,000-250,000 units/g solid (without added oxygen)
Sigma-Aldrich
Laccase from Agaricus bisporus, powder, deep brown, ≥4 U/mg
Sigma-Aldrich
Laccase from Trametes versicolor, powder, light brown, ≥0.5 U/mg