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In vivo phosphorylation of FtsZ2 in Arabidopsis thaliana.

The Biochemical journal (2012-07-25)
Daniela Gargano, Jodi Maple-Grødem, Simon G Møller
ABSTRACT

The tubulin-like FtsZ protein initiates assembly of the bacterial and plastid division machineries. In bacteria, phosphorylation of FtsZ impairs GTPase activity, polymerization and interactions with other division proteins. Using a proteomics approach, we have shown that AtFtsZ2 is phosphorylated in vivo in Arabidopsis and that PGK1 (phosphoglycerate kinase 1) interacts with AtFtsZ2 in planta, suggesting a possible role in FtsZ phosphorylation.

MATERIALS
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Sigma-Aldrich
3-Phosphoglyceric Phosphokinase from baker′s yeast (S. cerevisiae), ammonium sulfate suspension, ≥500 units/mg protein