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  • [Effect of univalent cations on synthesis of surfactants by Acinetobacter calcoaceticus IMV B-7241].

[Effect of univalent cations on synthesis of surfactants by Acinetobacter calcoaceticus IMV B-7241].

Mikrobiolohichnyi zhurnal (Kiev, Ukraine : 1993) (2013-06-01)
T P Pirog, T A Shevchuk, S I Antoniuk, E Iu Kravchenko, G A Iutinskaia
PMID23720959
ABSTRACT

The effect of univalent cations on activity of key enzymes of C2-metabolism has been investigated in the producer of biosurfactants, Acinetibacter calcoaceticus IMV B-7241 grown on ethanol. It was established that potassium cations are inhibitors of pyroquinolinequinone-dependent alcohol- and acetaldehyde dehydrogenases, the enzymes of biosynthesis of surface-active aminolipids (NADP-dependent glutamate dehydrogenase) and glycolipids (phosphoenopyruvate (PhEP)-carboxikinase), while ammonium cations are activators of these enzymes and PhEP-carboxylase. A decrease of potassium cations concentration in the cultivation medium to 1 mM and increase of the content of amine nitrogen to 10 mM as a result of potassium nitrate substitution by equimolar, as to nitrogen, urea concentration were accompanied by the increase of activity of enzymes of ethanol metabolism and SAS biosynthesis, as well as by the 2-fold increase of conditional concentration of the biosurfactants.

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