- Expression of recombinant platelet-derived endothelial cell growth factor in the yeast Saccharomyces cerevisiae.
Expression of recombinant platelet-derived endothelial cell growth factor in the yeast Saccharomyces cerevisiae.
Yeast (Chichester, England) (1992-01-01)
C Finnis, A Goodey, M Courtney, D Sleep
PMID1580101
ABSTRACT
A platelet-derived endothelial cell growth factor cDNA has been cloned, sequenced and expressed using the Saccharomyces cerevisiae PRB1 promoter. Soluble recombinant platelet-derived endothelial cell growth factor constituted 0.5-1.0% of total soluble protein. Yeast soluble protein extracts containing recombinant platelet-derived endothelial cell growth factor stimulate the growth of calf pulmonary artery endothelial cells in vitro.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Thymidine Phosphorylase, recombinant from Escherichia coli, recombinant, expressed in E. coli, buffered aqueous solution, ≥500 units/mL
Sigma-Aldrich
Thymidine Phosphorylase, recombinant from Escherichia coli, recombinant, expressed in E. coli, buffered aqueous solution, ≥900 units/mL, 0.2 μm filtered