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  • A screening system for enantioselective enzymes based on differential cell growth.

A screening system for enantioselective enzymes based on differential cell growth.

Chemical communications (Cambridge, England) (2002-07-20)
Manfred T Reetz, Carsten J Rüggeberg
ABSTRACT

The esterase-catalyzed enantioselective hydrolysis of the fluoroacetate of pantolactone leads to fluoroacetic acid, a toxic compound which inhibits the growth of esterase-producing yeast; this forms the basis of an ee-assay.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
D-(−)-Pantolactone, 99%
Sigma-Aldrich
DL-α-Hydroxy-β,β-dimethyl-γ-butyrolactone, purum, ≥97.0% (T)
Sigma-Aldrich
(S)-(+)-Pantolactone, 97%