- An interface chip connection between capillary electrophoresis and thermal lens microscope.
An interface chip connection between capillary electrophoresis and thermal lens microscope.
Electrophoresis (2003-03-26)
Kenji Uchiyama, Akihide Hibara, Kiichi Sato, Hideaki Hisamoto, Manabu Tokeshi, Takehiko Kitamori
PMID12652589
ABSTRACT
A thermal lens microscope (TLM) detection of capillary electrophoresis (CE) utilizing microchip technology was developed. Fused-silica capillaries with an inner diameter of 50 microm were directly connected to a microchannel in a microchip. The detection limit by TLM was estimated as 2.8 x 10(-7) absorbance by measuring pure water. The detection limit of derivatized amino acids determined by CE-TLM was estimated as 2.4 x 10(-8) M, which was 100 times lower than that of conventional absorbance detection.
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