[Solid phase microextraction and derivatization of sulfide in blood with pentafluorobenzylbromide].

Solid phase microextraction (SPME) and derivatization with pentafluorobenzylbromide for sulfide in blood was presented in the paper. The derivative of sulfide was extracted for 20 min at the condition of room temperature by a polydimethylsiloxane fiber. 1,3,5-tribromobenzene (IS) was used as an internal standerd. GC/MS was used for analysis. The ions at m/z 394 (derivative of sulfide) and m/z 312 (IS) in the selected ion minitoring chromatograms were selected for quantitation. The compound was detectable at least 0.01 microgram/ml. By the determination in poisoning blood, this method would be demonstrated to be simple.