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Molecular cloning and characterization of murine interleukin-11.

Experimental hematology (1996-10-01)
J C Morris, S Neben, F Bennett, H Finnerty, A Long, D R Beier, S Kovacic, J M McCoy, E DiBlasio-Smith, E R La Vallie, A Caruso, J Calvetti, G Morris, N Weich, S R Paul, P S Crosier, K J Turner, C R Wood
PMID8913282
ABSTRACT

Human interleukin-11 (IL-11) has been shown to have pleiotropic action on hematopoietic, hepatic, stromal, epithelial, neural, and osteoclast cells. In the present work, the murine IL-11 cDNA has been isolated from a fetal thymic cell line, and its structure and function compared with human IL-11. The murine protein was demonstrated to have identical actions on the proliferation of a murine plasmacytoma cell line, murine primitive bone marrow progenitor cells, and megakaryocyte precursors. The murine IL-11 protein was synthesized as a soluble thioredoxin-IL-11 fusion in Escherichia coli and the expression of murine IL-11 was examined by pulse-chase radiolabeling in COS cells. The chromosomal location of the murine IL-11 gene was assigned to the proximal arm of chromosome 7.