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  • [Interaction of chaotropically modified immunoglobulins with protein and glicolipid antigens].

[Interaction of chaotropically modified immunoglobulins with protein and glicolipid antigens].

Ukrains'kyi biokhimichnyi zhurnal (1999 ) (2007-05-15)
A I Gordienko, N V Khimich
ABSTRACT

The features of interaction of native and chaotropically modified immunoglobulins with proteins (ovalbumin) and glicolipids (lipopolysaccharides, LPS) enterobacteria Escherichia coli K235, Salmonella minnesota and Salmonella enteritidis have been investigated. It has been established, that after processing of native antibodies with 3.5 M KSCN their ability to contact to the specified antigenes repeatedly grows. Besides the intensity of interaction of modified immunoglobulins with the mentioned above antigenes was various, that is determined by the presence of structural distinctions between antigen determinants of proteins and glycolipid antigens, and also between O-polysaccharide chains of LPS in different species of enterobacteria.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Potassium thiocyanate, BioXtra, ≥99.0%
Sigma-Aldrich
Potassium thiocyanate, ACS reagent, 99%
Sigma-Aldrich
Potassium thiocyanate, ReagentPlus®, ≥99.0%
Sigma-Aldrich
Potassium thiocyanate solution, volumetric, 8 M KSCN