- Subfemtomole level protein sequencing by Edman degradation carried out in a microfluidic chip.
Subfemtomole level protein sequencing by Edman degradation carried out in a microfluidic chip.
Chemical communications (Cambridge, England) (2007-06-15)
Wenzhang Chen, Xuefeng Yin, Jinxia Mu, Yan Yin
PMID17563805
ABSTRACT
A novel microfluidic chip based Edman degradation system is developed, in which Edman degradation can be carried out with peptide at a subfemtomole level; combined with MALDI-TOF-MS detection, the identification specificity of gel-separated protein in low abundance has been drastically improved.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Phenyl isothiocyanate, Sigma Grade, 8.36 M, suitable for solid phase protein sequencing analysis, ≥99% (GC), liquid
Sigma-Aldrich
Phenyl isothiocyanate, for HPLC derivatization, the detection of alcohols and amines, ≥99.0%