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  • The Rv1712 Locus from Mycobacterium tuberculosis H37Rv codes for a functional CMP kinase that preferentially phosphorylates dCMP.

The Rv1712 Locus from Mycobacterium tuberculosis H37Rv codes for a functional CMP kinase that preferentially phosphorylates dCMP.

Journal of bacteriology (2009-02-03)
Caroline Thum, Cristopher Z Schneider, Mario S Palma, Diógenes S Santos, Luiz A Basso
ABSTRACT

The Mycobacterium tuberculosis cmk gene, predicted to encode a CMP kinase (CMK), was cloned and expressed, and its product was purified to homogeneity. Steady-state kinetics confirmed that M. tuberculosis CMK is a monomer that preferentially phosphorylates CMP and dCMP by a sequential mechanism. A plausible role for CMK is discussed.

MATERIALS
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Product Description

Sigma-Aldrich
2′-Deoxycytidine 5′-monophosphate, Sigma Grade, ≥95.0%