- Adipogenic differentiation of human induced pluripotent stem cells: comparison with that of human embryonic stem cells.
Adipogenic differentiation of human induced pluripotent stem cells: comparison with that of human embryonic stem cells.
FEBS letters (2009-03-03)
Daisuke Taura, Michio Noguchi, Masakatsu Sone, Kiminori Hosoda, Eisaku Mori, Yohei Okada, Kazutoshi Takahashi, Koichiro Homma, Naofumi Oyamada, Megumi Inuzuka, Takuhiro Sonoyama, Ken Ebihara, Naohisa Tamura, Hiroshi Itoh, Hirofumi Suemori, Norio Nakatsuji, Hideyuki Okano, Shinya Yamanaka, Kazuwa Nakao
PMID19250937
ABSTRACT
Induced pluripotent stem (iPS) cells were recently established from human fibroblasts. In the present study we investigated the adipogenic differentiation properties of four human iPS cell lines and compared them with those of two human embryonic stem (ES) cell lines. After 12 days of embryoid body formation and an additional 10 days of differentiation on Poly-l-ornithine and fibronectin- coated dishes with adipogenic differentiation medium, human iPS cells exhibited lipid accumulation and transcription of adipogenesis-related molecules such as C/EBPalpha, PPARgamma2, leptin and aP2. These results demonstrate that human iPS cells have an adipogenic potential comparable to human ES cells.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Poly-L-ornithine solution, mol wt 30,000-70,000, 0.01%, sterile-filtered, BioReagent, suitable for cell culture