The effect of levocabastine hydrochloride on human Tenon's capsule fibroblasts: inhibition of proliferation, suppression of DNA synthesis and induction of apoptosis.

To investigate the effects of levocabastine hydrochloride on the profileration, Deoxyribonucleic acid (DNA) synthesis and induction of apoptosis, in human Tenon's capsule fibroblasts. The maximum intoxicate concentration was determined by measuring the release of lactate dehydrogenase. Proliferation of fibroblasts was analyzed with a WST-1 method. DNA synthesis was examined by quantifying the incorporation of 5-bromo-2'-deoxyuridine into dividing cells, and apoptosis was analyzed using an Annexin-V-FLUOS Staining Kit. Levocabastine hydrochloride (10(-4) M), ketotifen fumarate (3 x 10(-5) M) and tranilast (10(-4) M) significantly inhibited proliferation of fibroblasts. Levocabastine hydrochloride (10(-4) M), ketotifen fumarate (10(-4) M) and tranilast (10(-3) M) inhibited DNA synthesis and induced apoptosis. Levocabastine hydrochloride inhibited the proliferation of fibroblasts.