A nano-confined charged layer defies the principle of electrostatic interaction.

The reactivity between two charged molecules and the activity of charged biomolecules are mainly governed by the principle of electrostatic interaction, i.e., like charges repel and opposite charges attract. In the present study it is shown that the principle of electrostatic interaction is violated in the nano-confined biomimetic environment. Thus a positively charged molecule shows more preference to a positively charged surface compared to a negatively charged surface.