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  • Efficacy of pentane, toluene, and benzene to support aerobic cometabolism of ethylene dibromide.

Efficacy of pentane, toluene, and benzene to support aerobic cometabolism of ethylene dibromide.

New biotechnology (2012-05-23)
Anthony S Danko, Patrícia O Leitão, Matthew F Verce, David L Freedman
ABSTRACT

The ability of pentane, benzene, and toluene to support aerobic cometabolism of ethylene dibromide (1,2-dibromoethane, EDB) was evaluated. A pentane enrichment culture cometabolized EDB, with a transformation capacity of 0.35 μmol EDB/mg biomass (66.2 μg EDB/mg biomass) in the absence of growth substrate. It also cometabolized EDB while actively growing on pentane. However, enrichment cultures grown on benzene or toluene could not cometabolize EDB, with or without their respective growth substrates.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Pentane, anhydrous, ≥99%
Sigma-Aldrich
1,2-Dichloroethane, anhydrous, 99.8%
Sigma-Aldrich
1,2-Dichloroethane, ACS reagent, ≥99.0%
Sigma-Aldrich
Pentane, ≥99% (GC)
Sigma-Aldrich
Pentane, reagent grade, 98%
Supelco
Pentane, analytical standard
Supelco
1,2-Dichloroethane, analytical standard
Supelco
1,2–Dichloroethane solution, certified reference material, 200 μg/mL in methanol
Supelco
1,2-Dibromoethane solution, certified reference material, 200 μg/mL in methanol
Sigma-Aldrich
Pentane, suitable for HPLC, ≥99.0%
Sigma-Aldrich
Pentane, spectrophotometric grade, ≥99%
Sigma-Aldrich
Pentane, puriss. p.a., ≥99.0% (GC)