Merck
CN
  • Efficient 2,3-butanediol production from cassava powder by a crop-biomass-utilizer, Enterobacter cloacae subsp. dissolvens SDM.

Efficient 2,3-butanediol production from cassava powder by a crop-biomass-utilizer, Enterobacter cloacae subsp. dissolvens SDM.

PloS one (2012-07-14)
Ailong Wang, Youqiang Xu, Cuiqing Ma, Chao Gao, Lixiang Li, Yu Wang, Fei Tao, Ping Xu
ABSTRACT

2,3-Butanediol (BD) is considered as one of the key platform chemicals used in a variety of industrial applications. It is crucial to find an efficient sugar-utilizing strain and feasible carbon source for the economical production of BD. Efficient BD production by a newly isolated Enterobacter cloacae subsp. dissolvens SDM was studied using crop-biomass cassava powder as substrate. The culture conditions and fermentation medium for BD production were optimized. Under the optimal conditions, 78.3 g l(-1) of BD was produced after 24 h in simultaneous saccharification and fermentation (SSF), with a yield of 0.42 g BD g(-1) cassava powder and a specific productivity of 3.3 g l(-1) h(-1). A higher BD concentration (93.9 g l(-1)) was produced after 47 h in fed-batch SSF. The results suggest that strain SDM is a good candidate for the BD production, and cassava powder could be used as an alternative substrate for the efficient production of BD.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
2,3-Butanediol, Vetec, reagent grade, 98%
Sigma-Aldrich
(2R,3R)-(−)-2,3-Butanediol, 97%
Sigma-Aldrich
2,3-Butanediol, 98%
Sigma-Aldrich
(2S,3S)-(+)-2,3-Butanediol, 97%
Sigma-Aldrich
meso-2,3-Butanediol, 99%