DNA duplex stabilization in crowded polyanion solutions.

DNA duplex stabilization in crowded polyanion solutions.

Chemical communications (Cambridge, England) (2013-01-11)

Imran Khimji, Jeehae Shin, Juewen Liu

ABSTRACT

The melting temperature of duplex DNA is much higher in polyanions than in non-ionic polymers with similar ionic strength, suggesting an additional electrostatic contribution on top of the excluded volume effect.

MATERIALS

Product Number

Brand

Product Description

523925

Sigma-Aldrich

Poly(acrylic acid) solution, average M_w ~100,000, 35 wt. % in H₂O

535931

Sigma-Aldrich

Poly(acrylic acid) solution, average M_w ~2,000, 50 wt. % in H₂O, electronic grade

416002

Sigma-Aldrich

Poly(acrylic acid) solution, average M_w ~250,000, 35 wt. % in H₂O

416010

Sigma-Aldrich

Poly(acrylic acid, sodium salt) solution, average M_w ~1,200, 45 wt. % in H₂O

416029

Sigma-Aldrich

Poly(acrylic acid, sodium salt) solution, average M_w ~8,000, 45 wt. % in H₂O

81117

Supelco

Poly(acrylic acid sodium salt), analytical standard, suitable for gel permeation chromatography (GPC), 1,250

81123

Supelco

Poly(acrylic acid sodium salt), analytical standard, suitable for gel permeation chromatography (GPC), 16,000

323667

Sigma-Aldrich

Poly(acrylic acid)

181293

Supelco

Poly(acrylic acid), analytical standard, average M_n 130,000 (Typical)

306231

Sigma-Aldrich

Poly(acrylic acid), average M_v ~4,000,000

306215

Sigma-Aldrich

Poly(acrylic acid), average M_v ~1,250,000

306223

Sigma-Aldrich

Poly(acrylic acid), average M_v ~3,000,000

420344

Sigma-Aldrich

Poly(acrylic acid sodium salt), average M_w ~2,100

181285

Sigma-Aldrich

Poly(acrylic acid), average M_v ~450,000

447013

Sigma-Aldrich

Poly(acrylic acid sodium salt), average M_w ~5,100 by GPC, powder