Merck
CN
  • A biopharmaceutical plant on a chip: continuous micro-devices for the production of monoclonal antibodies.

A biopharmaceutical plant on a chip: continuous micro-devices for the production of monoclonal antibodies.

Lab on a chip (2013-02-16)
Lucía D Garza-García, Leydi M Carrillo-Cocom, Diana Araiz-Hernández, Pedro Soto-Vázquez, Julián López-Meza, Eduardo J Tapia-Mejía, Sergio Camacho-León, Erika García-López, Ciro A Rodríguez-González, Mario M Alvarez
PMID23412111
ABSTRACT

We report a proof-of-principle for the use of micro-devices as continuous bioreactors for the production of monoclonal antibodies. We culture CHO cells on the surface of PMMA "zigzag" channels textured with semi-spherical cavities coated with fibronectin, observing steady-state productivities 100 times higher than those observed in full scale systems.

MATERIALS
Product Number
Brand
Product Description
03558
Supelco
Poly(methyl methacrylate), analytical standard, for GPC, 2,480,000
81512
Supelco
Poly(methyl methacrylate), analytical standard, for GPC, 8,000
370037
Supelco
Poly(methyl methacrylate), analytical standard, for GPC, average Mw 97,000 (Typical), average Mn 46,000 (Typical)
200336
Sigma-Aldrich
Poly(methyl methacrylate), average Mw ~15,000 by GPC, powder
81497
Supelco
Poly(methyl methacrylate), analytical standard, for GPC, 10,000
81501
Supelco
Poly(methyl methacrylate), analytical standard, for GPC, 50,000
81498
Supelco
Poly(methyl methacrylate), analytical standard, for GPC, 20,000
81489
Supelco
Poly(methyl methacrylate), analytical standard, for GPC, 2,000
445746
Sigma-Aldrich
Poly(methyl methacrylate), average Mw ~350,000 by GPC
81503
Supelco
Poly(methyl methacrylate), analytical standard, for GPC, 100,000
182265
Sigma-Aldrich
Poly(methyl methacrylate)
182230
Sigma-Aldrich
Poly(methyl methacrylate)
94131
Supelco
Poly(methyl methacrylate), analytical standard, for GPC, 4,000
452130
Sigma-Aldrich
Poly(methyl methacrylate), isotactic, >80% isotactic