- Autofluorescence generation and elimination: a lesson from glutaraldehyde.
Autofluorescence generation and elimination: a lesson from glutaraldehyde.
Chemical communications (Cambridge, England) (2013-03-07)
Kwahun Lee, Sungmoon Choi, Chun Yang, Hai-Chen Wu, Junhua Yu
PMID23462727
ABSTRACT
Glutaraldehyde causes especially high autofluorescence. It reacted with proteins and peptides to generate visible to near-IR emitters. A model indicated that ethylenediamine and a secondary amine in the molecule were key components for the formation of emissive species. The mechanism enables us to control the generation and elimination of autofluorescence.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 70% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 25% in H2O, specially purified for use as an electron microscopy fixative
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 50% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
Sigma-Aldrich
Glutaraldehyde solution, Grade I, 8% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use