Merck
CN

[Use of alcian green in morphological and histochemical problems].

Gegenbaurs morphologisches Jahrbuch (1984-01-01)
M Krutsay
ABSTRACT

With hydrochloric acid-alcoholic solution of alcian green, without any pretreatment both the sulfated and the carboxylated mucopolysaccharides (acid glycosaminoglycans) can be demonstrated. After an indirect sulfation (periodic acid-bisulfite pretreatment), the glycogen and the mucoproteins, and after an oxydation with acidified potassium permanganate, the high cystine-containing proteins can also be stained. As a counterstain, a picric acid-Sirius red solution has proved good.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Potassium disulfite, puriss., meets analytical specification of Ph.Eur., BP, NF, FCC, E224, 95.0-101.0% (iodometric), powder, 51.8-57.6% sulfur dioxide
Sigma-Aldrich
Potassium disulfite, ≥98%
Sigma-Aldrich
Potassium disulfite, BioUltra, ≥97.0% (RT)