Immunocytochemical study of the CLIP/ACTH-immunoreactive nerve fibres in the dorsal raphe nucleus of the rat.

The injection of corticotrophin-like intermediate lobe peptide (CLIP) in the lateral ventricle or the dorsal raphe nucleus (DRN) of the rat is followed by a significant increase in the amount of paradoxical sleep. In the DRN, CLIP would act through a somatic and/or dendritic release of serotonin. To establish the anatomical basis of these effects, the nerve fibres immunoreactive for CLIP/ACTH were labelled and their fine anatomical relationships with the neuronal elements of the DRN were studied at the ultrastructural level. Half of the profiles of labelled varicosities were 'free' in the neuropile, the other half was in close contact with dendrites, either shafts or spines. It was interesting to note that few contacts with dendritic shafts were under the form of a synapse, whereas a large number of the contacts with spines were synaptic. The chemical nature of these dendritic targets remains to be determined but preliminary results indicate that they are partly serotoninergic.