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Biosynthetic pathway for the C45 polyprenol, solanesol, in tobacco.

Bioscience, biotechnology, and biochemistry (2004-09-25)
Ei'ichiro Fukusaki, Shinya Takeno, Takeshi Bamba, Hiroshi Okumoto, Hiroko Katto, Shin'ichiro Kajiyama, Akio Kobayashi
ABSTRACT

Feeding experiments were independently performed with [1-13C]deoxy-D-xylulose triacetate and (RS)-[2-13C]mevalonolactone in the tobacco plant. The labeling pattern for solanesol was elucidated to reveal that the isoprene moiety of solanesol would be derived from deoxy-xylulose. The result strongly suggests that tobacco solanesol is biosynthesized via the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway.